Team:UGent/Attributions
From 2013.igem.org
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Pieter Coussement, Frederik De Bruyn and Gert Peters gave us an introduction to common lab techniques. Frederik also gave us a tutorial on Clone Manager. We received safety training from Gilles Velghe who also made us familiar with the lab. Isabelle Maryns showed us how to safely handle ethidiumbromide. Safety forms were filled in by the students and Prof. De Mey. | Pieter Coussement, Frederik De Bruyn and Gert Peters gave us an introduction to common lab techniques. Frederik also gave us a tutorial on Clone Manager. We received safety training from Gilles Velghe who also made us familiar with the lab. Isabelle Maryns showed us how to safely handle ethidiumbromide. Safety forms were filled in by the students and Prof. De Mey. | ||
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- | Inbio (the centre of expertise for industrial biotechnology and biocatalysis)let us make use of their lab and made sure all the necessary lab equipment was at our disposal. | + | Inbio (the UGent centre of expertise for industrial biotechnology and biocatalysis) let us make use of their lab and made sure all the necessary lab equipment was at our disposal. |
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- | Inbio provided us with the strain bearing plasmids that contain the ccdA-gfp construct we used (E. coli DH5a + pTGD-ccdA-Pmb1-BCD7-GFPCmFRT). They provided the bacterial strain in which the knock-in was performed (E. coli MG1655 | + | Inbio provided us with the strain bearing plasmids that contain the ccdA-gfp construct we used (E. coli DH5a + pTGD-ccdA-Pmb1-BCD7-GFPCmFRT). They provided the bacterial strain in which the knock-in was performed (E. coli MG1655 ΔendA DE3), strains containing plasmids with the T7-ccdB fragment (E. coli DH5a + pXSpFRT-T7ccdB) and the strain we used for making P1 phage lysate (E. coli BW26547), along with a small dose of P1 lysate. Inbio also constructed back-up strains (E. coli MG1655 #916endA DE3 ccdA-Pmb1GFP-CmFRT & E. coli MG1655 endA DE3 ccdA-Pmb1GFP-CmFRT) in which CIChE could be performed if other experiments failed. |
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Primers starting with MDM and CGL were designed by Prof. Marjan De Mey. Primers beginning with oMEMO were designed by Inbio. Primers starting with iGEM were designed by students of the UGent iGEM team. Pieter Coussement also designed some primers for a reverse mutation. | Primers starting with MDM and CGL were designed by Prof. Marjan De Mey. Primers beginning with oMEMO were designed by Inbio. Primers starting with iGEM were designed by students of the UGent iGEM team. Pieter Coussement also designed some primers for a reverse mutation. |
Revision as of 21:41, 3 October 2013
Prof. Marjan De Mey and Charlotte Verniers assisted us during brainstorming and advised us during our literature study and the writing and presentation of our paper. Frederik De Bruyn also threw in some ideas during brainstorming.
Pieter Coussement, Frederik De Bruyn and Gert Peters gave us an introduction to common lab techniques. Frederik also gave us a tutorial on Clone Manager. We received safety training from Gilles Velghe who also made us familiar with the lab. Isabelle Maryns showed us how to safely handle ethidiumbromide. Safety forms were filled in by the students and Prof. De Mey.
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