Team:UGent/Results

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<h1> Plasmids </h1>
<h1> Plasmids </h1>
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We constructed plasmid pSB6A1-T7ccdB for use in CIChE. Through this plasmid, pressure can be put on the CIChE strains simply by adding IPTG.
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We also purified plasmids p5SpFRT-T7ccdB, p10SpFRT-T7ccdB, p20SpFRT-T7ccdB from strains we obtained from Inbio. These plasmids have different copy numbers and they too can be used in chromosomal evolution.
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<h1> Strains </h1>
<h1> Strains </h1>
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<h1> CIChE </h1>
<h1> CIChE </h1>
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Revision as of 23:04, 3 October 2013

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Plasmids

We constructed plasmid pSB6A1-T7ccdB for use in CIChE. Through this plasmid, pressure can be put on the CIChE strains simply by adding IPTG.

We also purified plasmids p5SpFRT-T7ccdB, p10SpFRT-T7ccdB, p20SpFRT-T7ccdB from strains we obtained from Inbio. These plasmids have different copy numbers and they too can be used in chromosomal evolution.

Strains

CIChE

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Bio Base Europe Pilot Plant
Inbio
Bioké Novolab
MRP UGent