Team:UGent/Results
From 2013.igem.org
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We also purified plasmids p5SpFRT-T7ccdB, p10SpFRT-T7ccdB, p20SpFRT-T7ccdB from strains we obtained from Inbio. These plasmids have different copy numbers and they too can be used in chromosomal evolution. | We also purified plasmids p5SpFRT-T7ccdB, p10SpFRT-T7ccdB, p20SpFRT-T7ccdB from strains we obtained from Inbio. These plasmids have different copy numbers and they too can be used in chromosomal evolution. | ||
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- | + | Having plasmids with different copy numbers at our disposal, we can test CIChE with different degrees of toxin-pressure. | |
</p> | </p> | ||
<h1> Strains </h1> | <h1> Strains </h1> |
Revision as of 23:10, 3 October 2013
Plasmids
We constructed plasmid pSB6A1-T7ccdB for use in CIChE. Through this plasmid, pressure can be put on the CIChE strains simply by adding IPTG.
Strains
CIChE
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