Team:UGent/Results
From 2013.igem.org
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The following strain containing the construct to be duplicated (<i>ccdA-gfp</i>) was constructed (KI experiment 1):</p> | The following strain containing the construct to be duplicated (<i>ccdA-gfp</i>) was constructed (KI experiment 1):</p> | ||
- | <p><center><i>E. coli</i> MG1655 ΔendA DE3 ccdA-Pmb1GFP-CmFRT (called strain 8 | + | <p><center><i>E. coli</i> MG1655 ΔendA DE3 ccdA-Pmb1GFP-CmFRT (called strain 8)</center></p> <p> |
We also dispose of a backup version of this strain, constructed by Inbio in case our own experiment failed (called MDM.Cm). We included this second version in our further experiments as to test possible differences between the two versions. | We also dispose of a backup version of this strain, constructed by Inbio in case our own experiment failed (called MDM.Cm). We included this second version in our further experiments as to test possible differences between the two versions. | ||
</p> | </p> | ||
<h1> CIChE Strains</h1> | <h1> CIChE Strains</h1> | ||
<p> | <p> | ||
- | + | The plasmids and strains mentioned above were used in transformations (experiment 3) to obtain strains containing all elements necessary to perform chromosomal evolution. The following strains were constructed:<br> | |
+ | 8+pSB6A1-T7ccdB <br> | ||
+ | 8+p5SpFRT-T7ccdB <br> | ||
+ | 8+p10SpFRT-T7ccdB <br> | ||
+ | 8+p20SpFRT-T7ccdB <br> | ||
</p> | </p> | ||
<h1> CIChE </h1> | <h1> CIChE </h1> |
Revision as of 23:58, 3 October 2013
Plasmids containing T7-ccdB
We constructed plasmid pSB6A1-T7ccdB for use in CIChE. Through this plasmid, pressure can be put on the CIChE strains simply by adding IPTG.
Strains with ccdA-gfp constructThe following strain containing the construct to be duplicated (ccdA-gfp) was constructed (KI experiment 1): We also dispose of a backup version of this strain, constructed by Inbio in case our own experiment failed (called MDM.Cm). We included this second version in our further experiments as to test possible differences between the two versions. CIChE Strains
The plasmids and strains mentioned above were used in transformations (experiment 3) to obtain strains containing all elements necessary to perform chromosomal evolution. The following strains were constructed: CIChE
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