"
Team:UGent/CloneManager
From 2013.igem.org
(Difference between revisions)
| Line 14: | Line 14: | ||
<p> All of these plasmids are constucted using biobrick-standard parts: the plasmid backbones pSB3T5, pSB4A5 and pSB6A1. The <i> ccdB </i> gene that encodes the toxin is controlled by T7 promotor. </p> | <p> All of these plasmids are constucted using biobrick-standard parts: the plasmid backbones pSB3T5, pSB4A5 and pSB6A1. The <i> ccdB </i> gene that encodes the toxin is controlled by T7 promotor. </p> | ||
| - | <h2>pSB3T5 T7 ccdB</h2> | + | <h2>pSB3T5 T7 <i> ccdB </i></h2> |
<center><img src="https://static.igem.org/mediawiki/2013/4/4a/UGent_2013_PSB3T5-T7-ccdB.png" width="500"/></center> | <center><img src="https://static.igem.org/mediawiki/2013/4/4a/UGent_2013_PSB3T5-T7-ccdB.png" width="500"/></center> | ||
| - | <h2>pSB4A5 T7 ccdB</h2> | + | <h2>pSB4A5 T7 <i> ccdB </i></h2> |
<center><img src="https://static.igem.org/mediawiki/2013/b/bc/UGent_2013_PSB4A5-T7-ccdB.png" width="500"/></center> | <center><img src="https://static.igem.org/mediawiki/2013/b/bc/UGent_2013_PSB4A5-T7-ccdB.png" width="500"/></center> | ||
| - | <h2>pSB6A1 T7 ccdB</h2> | + | <h2>pSB6A1 T7 <i> ccdB </i></h2> |
<center><img src="https://static.igem.org/mediawiki/2013/b/b1/UGent_2013_PSB6A1-T7-ccdB.png" width="500"/></center> | <center><img src="https://static.igem.org/mediawiki/2013/b/b1/UGent_2013_PSB6A1-T7-ccdB.png" width="500"/></center> | ||
<h1> Plasmid containing new part </h1> | <h1> Plasmid containing new part </h1> | ||
Revision as of 02:09, 4 October 2013
 |
|
|
To perform in silico analysis, we used Clone Manager. All plasmids were first implemented into the software, and then used to help with cloning simulation, restriction, PCR etc. Plasmid containing construct for chromosomal evolutionpTGD ccdA Pmb1 BCD7 GFP CmFRTThis plasmid contains the ccdA antitoxin and the reporter gene gfp. The homologous regions are necessary to perform Chemically Inducible Chromosomal evolution (CIChE). The removal of the chloramphenicol resistance cassette between the FTR sites is done using the method of Datsenko & Wanner [PNAS 2000].  Plasmids containing toxin ccdBAll of these plasmids are constucted using biobrick-standard parts: the plasmid backbones pSB3T5, pSB4A5 and pSB6A1. The ccdB gene that encodes the toxin is controlled by T7 promotor. pSB3T5 T7 ccdB pSB4A5 T7 ccdB pSB6A1 T7 ccdB Plasmid containing new partpSB1C3 T7 ccdB
|
Tweets van @iGEM_UGent |
|
|
