Team:UGent/Results
From 2013.igem.org
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Revision as of 02:18, 4 October 2013
Plasmids containing T7-ccdB![]()
We constructed plasmid pSB6A1-T7ccdB for use in CIChE. Through this plasmid, pressure can be put on the CIChE strains simply by adding IPTG.
Strains with ccdA-gfp constructThe following strain containing the construct to be duplicated (ccdA-gfp) was constructed (KI experiment 1): We also dispose of a backup version of this strain, constructed by Inbio in case our own experiment failed (called MDM.Cm). We included this second version in our further experiments as to test possible differences between the two versions. CIChE Strains
The plasmids and strains mentioned above were used in transformations (experiment 3) to obtain strains containing all elements necessary to perform chromosomal evolution. The following strains were constructed:
UV Test Phage transduction was used to perform the deletion of the recA gene. To check whether the gene was successfully knocked out, an UV test was developed. The UV light puts a certain amount of stress on the bacterial cells. Due to this stress, an SOS pathway is triggered in which recA plays an important role. Cells in which the recA gene is deleted will not survive on the backup plate.
CIChE
Recommendations for further research
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![]() Tweets van @iGEM_UGent |
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