Team:Imperial College/mainresults
From 2013.igem.org
Line 78: | Line 78: | ||
|[[File:743px-3HB_assay_from_PHB_másolata.jpg|thumbnail|left|600px| The chemical analysis of the produced bioplastic. The samples break break down to 3HB monomers after treatment with our PhaZ1 enzyme (BBa_K1149010). We synthesised P(3HB) using our improved Biobrick part (hybrid promoter phaCAB, BBa_K1149051). Our engineered bioplastic producing <i>E.coli</i> synthesised P(3HB) directly from waste. Imperial iGEM data]] | |[[File:743px-3HB_assay_from_PHB_másolata.jpg|thumbnail|left|600px| The chemical analysis of the produced bioplastic. The samples break break down to 3HB monomers after treatment with our PhaZ1 enzyme (BBa_K1149010). We synthesised P(3HB) using our improved Biobrick part (hybrid promoter phaCAB, BBa_K1149051). Our engineered bioplastic producing <i>E.coli</i> synthesised P(3HB) directly from waste. Imperial iGEM data]] | ||
|} | |} | ||
- | + | ||
+ | {| class="wikitable" style="margin: 1em auto 1em auto;" | ||
+ | |[[File:Imperial_P3HB_from_waste.jpg|thumbnail|left|600px|<b>Degradation of P3HB produced from various sources showing no significant difference between P3HB made from waste and P3HB made from other sources.</b>]] | ||
+ | |} | ||
<html> | <html> | ||
Line 94: | Line 97: | ||
</div> | </div> | ||
<div class="TabbedPanelsContent"> | <div class="TabbedPanelsContent"> | ||
- | |||
<div id="CollapsiblePanelM21" class="CollapsiblePanel"> | <div id="CollapsiblePanelM21" class="CollapsiblePanel"> | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
<div class="CollapsiblePanelTab" tabindex="0"><h4>We degraded P3HB </html><font size="1">▼</font size="1"><html></h4></div> | <div class="CollapsiblePanelTab" tabindex="0"><h4>We degraded P3HB </html><font size="1">▼</font size="1"><html></h4></div> | ||
<div class="CollapsiblePanelContent"> | <div class="CollapsiblePanelContent"> | ||
Line 124: | Line 113: | ||
</div> | </div> | ||
- | <div id=" | + | <div id="CollapsiblePanelM22" class="CollapsiblePanel"> |
<div class="CollapsiblePanelTab" tabindex="0"><h4>We degraded P3HB we made from waste </html><font size="1">▼</font size="1"><html></h4></div> | <div class="CollapsiblePanelTab" tabindex="0"><h4>We degraded P3HB we made from waste </html><font size="1">▼</font size="1"><html></h4></div> | ||
<div class="CollapsiblePanelContent"> | <div class="CollapsiblePanelContent"> | ||
Line 131: | Line 120: | ||
<p align="justify">Using [https://www.caymanchem.com/app/template/Product.vm/catalog/700190 3HB colourimetric assay kit], we have shown that we have degraded the P3HB made from waste into 3HB monomers. In addition, there is no significant difference in 3HB concentration between different P3HB sources. This result proves that <b>we now have a closed loop for P3HB bioplastic recycling!</b></p> | <p align="justify">Using [https://www.caymanchem.com/app/template/Product.vm/catalog/700190 3HB colourimetric assay kit], we have shown that we have degraded the P3HB made from waste into 3HB monomers. In addition, there is no significant difference in 3HB concentration between different P3HB sources. This result proves that <b>we now have a closed loop for P3HB bioplastic recycling!</b></p> | ||
+ | |||
+ | {| class="wikitable" style="margin: 1em auto 1em auto;" | ||
+ | |[[File:Imperial_P3HB_from_waste.jpg|thumbnail|left|600px|<b>Degradation of P3HB produced from various sources showing no significant difference between P3HB made from waste and P3HB made from other sources.</b>]] | ||
+ | |} | ||
Line 137: | Line 130: | ||
</div> | </div> | ||
- | <div id=" | + | <div id="CollapsiblePanelM23" class="CollapsiblePanel"> |
<div class="CollapsiblePanelTab" tabindex="0"><h4>We degraded PLA </html><font size="1">▼</font size="1"><html></h4></div> | <div class="CollapsiblePanelTab" tabindex="0"><h4>We degraded PLA </html><font size="1">▼</font size="1"><html></h4></div> | ||
<div class="CollapsiblePanelContent">Content | <div class="CollapsiblePanelContent">Content | ||
Line 159: | Line 152: | ||
var CollapsiblePanelM21 = new Spry.Widget.CollapsiblePanel("CollapsiblePanelM21", {contentIsOpen:false}); | var CollapsiblePanelM21 = new Spry.Widget.CollapsiblePanel("CollapsiblePanelM21", {contentIsOpen:false}); | ||
- | var CollapsiblePanelM22 = new Spry.Widget.CollapsiblePanel("CollapsiblePanelM22", { | + | var CollapsiblePanelM22 = new Spry.Widget.CollapsiblePanel("CollapsiblePanelM22", {contentIsClose:false}); |
var CollapsiblePanelM23 = new Spry.Widget.CollapsiblePanel("CollapsiblePanelM23", {contentIsClose:false}); | var CollapsiblePanelM23 = new Spry.Widget.CollapsiblePanel("CollapsiblePanelM23", {contentIsClose:false}); | ||
- | + | ||
Revision as of 19:53, 4 October 2013
Main Results
Resource-full Waste
Plastic Fantastic
We made P3HB bioplastic ▼
Modelling ▼
In order to realistically improve our experimental design, we produced P3HB synthesis model with considerations of central metabolic pathway within the cells. Our model predicts that during the synthesis of P3HB, the concentration of PhaB enzymes is an important rate limiting factor that can actually be regulated.
We optimised bioplastic production ▼
Since our model predicts that P3HB production can be significantly improved by expressing more P3HB polymerase PhaB, we designed a [http://parts.igem.org/Part:BBa_K1149051 hybrid promoter] consists of the J23104 constitutive promoter and the native promoter to optimise gene expression. Our results show that we have successfully produced 11-fold more P3HB bioplastic compared with the native promoter.
We made bioplastic from mixed waste ▼
One of the objectives of Module 1 is to produce P3HB bioplastic from waste. By comparing degradation product 3HB of P3HB bought from Sigma, produced from glucose and produced from the waste, we found there is no significant differences in 3HB concentration between these samples.
We degraded P3HB ▼
We degraded P3HB we made from waste ▼
Using 3HB colourimetric assay kit, we have shown that we have degraded the P3HB made from waste into 3HB monomers. In addition, there is no significant difference in 3HB concentration between different P3HB sources. This result proves that we now have a closed loop for P3HB bioplastic recycling!
We degraded PLA ▼