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Team:TU Darmstadt/materials/Buffers
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Revision as of 00:40, 5 October 2013
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Buffers
Tris
- Tris is a common buffering agent and is short for tris[hydroxymethyl]aminomethane (C4H11NO3, 2-Amino-2-hydroxymethyl-propane-1,3-diol).
1x PBS
- 8.18 g NaCl (140 mM)
- 0.2 g KCl (2.7 mM)
- 1.77 g Na2HPO4 (10 mM)
- 0.24 g KH2PO4 (1.8 mM)
- Add. 1L dH2O
- Adjust to pH 7.4
TE buffer
- 10 mM Tris, bring to pH 8.0 with HCl
- 1 mM EDTA (Ethylenediaminetetraacetic acid)
50x TAE
- 242g Tris base in water
- add 57.1mL glacial acetic acid
- 100mL of 500mM EDTA (pH 8.0) solution
- add up to 1 litre of water
