Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period2/Dailylog

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<font size="4"> '''7/2/13''' </font>
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<font size="4"> '''7/17/13''' </font>
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- Performed second round of titer for [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period3/Exp/6.27_Mutagen_Concentration_Test_-_Third_Protocol|6.27 Mutagen Concentration Test - Third Protocol]], repeating the procedures from last Friday.
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- The titer from 7.15 was too low to start an actual round of mutagenesis. One more round of propagation was performed for [[Team:BYU_Provo/Notebook/SmallPhage/Summer/Period1/Exp/7.13_Phage_Propagation]]
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- Started approximately 50mL of BL21 liquid culture overnight.
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- Do we want to talk about this round of mutagenesis? phage stock 80e6. 1.5mL of E coli, 90uL of phage.
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Revision as of 22:10, 17 July 2013


Small Phage July - August Notebook: July 16 - July 31 Daily Log



Overview
March-April
May-June
July-August
September-October

7/16/13

- Started approximately 60mL of BL21 liquid culture overnight.


7/17/13

- The titer from 7.15 was too low to start an actual round of mutagenesis. One more round of propagation was performed for Team:BYU_Provo/Notebook/SmallPhage/Summer/Period1/Exp/7.13_Phage_Propagation

- Do we want to talk about this round of mutagenesis? phage stock 80e6. 1.5mL of E coli, 90uL of phage.


7/3/13

- Performed spot test for 6.27 Mutagen Concentration Test - Third Protocol to determine phage viability. 5.3 stock and phage from 6.12 Mutagen Concentration Test - Second Protocol were used as positive control.

- Worked on the introduction for our outreach presentation.


7/7/13

- Started 60mL of BL21 liquid culture overnight.


7/8/13

- Made 750mL of x8 top agar.

- We started our fourth round of mutagenesis in 7.8 Mutagen Concentration Test - Fourth Protocol


7/9/13

- Outreach @ Orem Public Library!

- Started approximately 20mL of E coli BL21 liquid culture overnight.


7/10/13

- Performed titer to determine mutated phage concentration for 7.8 Mutagen Concentration Test - Fourth Protocol


7/11/13

- Started approximately 10mL of E coli BL21 liquid culture overnight.


7/12/13

- Performed spot test to confirm the titer result from July 10th. Detail procedure listed in 7.8 Mutagen Concentration Test - Fourth Protocol.

- Started approximately 6 mL of E coli liquid culture overnight.


7/13/13

- Set up the phage liquid culture for propagation in 7.13 Phage Propagation Experiment.


7/14/13

- Started approximately 30mL of E coli BL21 liquid culture overnight.