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From 2013.igem.org

Revision as of 11:15, 16 July 2013 (view source) Fentwistle (Talk | contribs) (→Afternoon) ← Older edit		Revision as of 10:02, 18 July 2013 (view source) Fentwistle (Talk | contribs) (→Afternoon) Newer edit →
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	- BBa_K592010, yellow pigment		- BBa_K592010, yellow pigment
		+
	- BBa_K592012, magenta pigment		- BBa_K592012, magenta pigment
		+
	- BBa_B0015, a terminator		- BBa_B0015, a terminator
		+
	- BBa_K592005, FixJ protein intermediate		- BBa_K592005, FixJ protein intermediate
		+
	- BBa_K592004, blue light sensor		- BBa_K592004, blue light sensor

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Revision as of 10:02, 18 July 2013

Morning

MiniPrep of the following Parts:

- BBa_K322122, cyan pigment

- BBa_K592012, magenta pigment

- BBa_K592010, yellow pigment

Plasmids extracted are now in storage at -20oC.

Afternoon

Liquid cultures of the blue light module and magenta pigment

- BBa_K592010, yellow pigment

- BBa_K592012, magenta pigment

- BBa_B0015, a terminator

- BBa_K592005, FixJ protein intermediate

- BBa_K592004, blue light sensor

Retry of BBa_B0034 transformation

Normal transformation protocol from 4/7/13 followed. Made certain we were using the right antibiotic plates!

(Next day, results of BBa_B0034 transformation)

We made two plates of BBa_B0034, each plate had 7 colonies. Our RFP control had 137 colonies.