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Team:TU-Munich/Notebook/Materials
From 2013.igem.org
(Difference between revisions)
(→Oligonucleotides) |
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| + | |xylE_for | ||
| + | |<code>5'- AAGCTTTCTAGATGGCCGGCAACAAAGGTGTAATGCGACCG - 3'</code><br> | ||
| + | |- | ||
| | | | ||
| - | |<code>5'- - 3'</code><br> | + | |xylE_rev |
| - | |- | + | |<code>5'- TTCGAAACCGGTGGTCAGCACGGTCATGAATC - 3'</code><br> |
| - | + | |- | |
| | | | ||
| - | |<code>5'- - 3'</code><br> | + | |xylE_c312a_for |
| + | |<code>5'- GAACAGTTGTGGACGGCGCGTGCGC - 3'</code><br> | ||
|- | |- | ||
| | | | ||
| - | | | + | |xylE_c312a_rev |
| - | |<code>5'- | + | |<code>5'- GCGCACGCGCCGTCCACAACTGTTC - 3'</code><br> |
|- | |- | ||
| | | | ||
| - | | | + | |xylE_g483a_for |
| - | |<code>5'- | + | |<code>5'- CCTCATGTATGGCGACGAATTACCGGCGACCTA - 3'</code><br> |
|- | |- | ||
| | | | ||
| - | | | + | |xylE_g483a_rev |
| - | |<code>5'- | + | |<code>5'- TAGGTCGCCGGTAATTCGTCGCCATACATGAGG - 3'</code><br> |
|- | |- | ||
| | | | ||
| - | | | + | |xylE_a834g_for |
| - | |<code>5'- | + | |<code>5'- CAACTACCCGGACCACAAGCCGGTGACCT - 3'</code><br> |
|- | |- | ||
| | | | ||
| - | | | + | |xylE_a834_rev |
| - | |<code>5'- | + | |<code>5'- AGGTCACCGGCTTGTGGTCCGGGTAGTTG - 3'</code><br> |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
|- | |- | ||
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Revision as of 19:17, 6 May 2013
Oligonucleotides
| Name | Sequence | |
| EreA_for | 5’- GCGCGTCTAGATGGCCGGCACGTGGAGAACGACCAGA -3‘ | |
| EreA-rev | 5‘- TTCGAAACCGGTTAGGGCAACTAGGCTGTCC -3‘ | |
| EreA_SDMI_for | 5’- CGAATCAGGAAGAAAACTACAGTTAGTCGGAATCGAC -3‘ | |
| EreA_SDMI_rev | 5'- GTCGATTCCGACTAACTGTAGTTTTCTTCCTGATTCG -3' | |
| EreA_SDMII_for | 5'- GACGCCTGTGGTACGGAAAATTCATGTCTGACATT - 3' | |
| EreA_SDMII_rev | 5'- AATGTCAGACATGAATTTTCCGTACCACAGGCGTC - 3' | |
| EreA_SDMIII_for | 5'- GTGTTCCAAGCGCGGGCAAGGACAGCC - 3' | |
| EreA_SDMIII_rev | 5'- GGCTGTCCTTGCCCGCGCTTGGAACAC - 3' | |
| EreB_for | 5'- GCGCGTCTAGATGGCCGGCAGGTTCGAAGAATGGGTC - 3' | |
| EreB-rev | 5'- TTCGAAACCGGTTTCATAAACGACCTCAGATACAG - 3' | |
| EreB_SDMI_for | 5'- GGTGAAAATTCTCATTTCATAAAAGAGTTCTTTTTGTTACGACATACGCTTTT - 3' | |
| EreB_SDMI_rev | 5'- AAAAGCGTATGTCGTAACAAAAAGAACTCTTTTATGAAATGAGAATTTTCACC - 3' | |
| EreB_SDMII_for | 5'- CTTATAGTGGGCATACAGCAGCCCTCTATCCGG - 3' | |
| EreB_SDMII_rev | 5'- CCGGATAGAGGGCTGCTGTATGCCCACTATAAG - 3' | |
| EreB_SDMIII_for | 5'- CGAGTTGATAACTTCCAACTACAGGAACCAAATGAAGGTTC - 3' | |
| EreB_SDMIII_rev | 5'- GAACCTTCATTTGGTTCCTGTAGTTGGAAGTTATCAACTCG - 3' | |
| TEV_fw | 5'- - 3' | |
| TEV_rv | 5'- - 3' | |
| TEV_t387c_fw | 5'- - 3' | |
| TEV_t387c_rv | 5'- - 3' | |
| N_split_TEV_fw | 5'- - 3' | |
| N_split_TEV_rv | 5'- - 3' | |
| C_split_TEV_fw | 5'- - 3' | |
| C_split_TEV_fw | 5'- - 3' | |
| PIF6_2-100_fw | 5'- - 3' | |
| PIF6_2-100_rv | 5'- - 3' | |
| MinMCS_fo | 5'- ctagacctaggaagcttcctgcaggactagtaagcttctgca - 3' | |
| MinMCS_rev | 5'- gaagcttactagtcctgcaggaagcttcctaggt - 3' | |
| Bpu_for | 5'- GCGCGTCTAGATGGCCGGCAACCTAGAAAAATTTGTTGACGAGCTG - 3' | |
| Bpu_rev | 5'- TTCGAAACCGGTGATGATATCCATCGGCCGCAT - 3' | |
| Bpu_SDMI_for | 5'- cacttaagcattttctacgggtcgatcacaccattca - 3' | |
| Bpu_SDMI_rev | 5'- tgaatggtgtgatcgacccgtagaaaatgcttaagtg - 3' | |
| Bpu_SDMII_fo | 5'- gacacgattaaatgtgtatgcgggcttagctggattttatttg - 3' | |
| Bpu_SDMII_re | 5'- caaataaaatccagctaagcccgcatacacatttaatcgtgtc - 3' | |
| Bpu_SDMIII_fo | 5'- cacccttaaaaataaagctggctgcggacaggaag - 3' | |
| Bpu_SDMIII_re | 5'- cttcctgtccgcagccagctttatttttaagggtg - 3' | |
| Bpu_SDMIV_fo | 5'- cgtacgctcactcttacgggtacacaggataaata - 3' | |
| Bpu_SDMIV_re | 5'- tatttatcctgtgtacccgtaagagtgagcgtacg - 3' | |
| xylE_for | 5'- AAGCTTTCTAGATGGCCGGCAACAAAGGTGTAATGCGACCG - 3' | |
| xylE_rev | 5'- TTCGAAACCGGTGGTCAGCACGGTCATGAATC - 3' | |
| xylE_c312a_for | 5'- GAACAGTTGTGGACGGCGCGTGCGC - 3' | |
| xylE_c312a_rev | 5'- GCGCACGCGCCGTCCACAACTGTTC - 3' | |
| xylE_g483a_for | 5'- CCTCATGTATGGCGACGAATTACCGGCGACCTA - 3' | |
| xylE_g483a_rev | 5'- TAGGTCGCCGGTAATTCGTCGCCATACATGAGG - 3' | |
| xylE_a834g_for | 5'- CAACTACCCGGACCACAAGCCGGTGACCT - 3' | |
| xylE_a834_rev | 5'- AGGTCACCGGCTTGTGGTCCGGGTAGTTG - 3' | |
5'- - 3' | ||
5'- - 3' | ||
5'- - 3' | ||
5'- - 3' | ||
5'- - 3' | ||
5'- - 3' | ||
5'- - 3' | ||
5'- - 3' | ||
5'- - 3' | ||
5'- - 3' | ||
5'- - 3' | ||
5'- - 3' | ||
5'- - 3' | ||
5'- - 3' |
