Team:UTK-Knoxville/6 June 2013

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<a href="../UTK-knoxville/2013.igem.org">2013.igem.org</a></p>
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<div id="header"><a href="https://2013.igem.org"><img src="https://static.igem.org/mediawiki/2013/thumb/2/20/Header2.jpg/800px-Header2.jpg" alt="Team UTK-Knoxville" width="910" height="234"></a></div>
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<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Overview</span></span></a></li>
 
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<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Overall Project</span></span></a></li>
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Overall Project</span></span></a></li>
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<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION3</span></span></a></li>
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<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION4</span></span></a></li>
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<h1><img src="https://static.igem.org/mediawiki/2013/a/a1/CalendarNAVI.png" alt="NotebookNAVI" width="485" height="144" border="0" usemap="#Map2">
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<h1><span class="mw-headline">June 06rd 2013</span></h1>
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<p>Ivan, Brandon, Eric, and Patrick met to discuss the RcoM, HemAT, and cytoplasmic primers and the wiki page.</p>
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<h3> <span class="mw-headline" id="Tuesday_06_June_13"> Tuesday 06 June 13 </span>     </h3>
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<li> Ivan, Brandon, Eric, and Patrick met to discuss the RcoM, HemAT, and cytoplasmic primers and the wiki page.</li>
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<p>Ligation Aer/EnvZ + OmpR + BB</p>
<p>Ligation Aer/EnvZ + OmpR + BB</p>
<p>3:1 - 1 uL BB, 1 uL Ligase, 1 uL Buffer, 1.75 uL OmpR, 1.75 uL Aer/EnvZ, 3.5 uL sterile DI water.</p>
<p>3:1 - 1 uL BB, 1 uL Ligase, 1 uL Buffer, 1.75 uL OmpR, 1.75 uL Aer/EnvZ, 3.5 uL sterile DI water.</p>
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<p>5:1 - 1 uL BB, 1 uL Ligase, 1 uL Buffer, 3 uL OmpR, 3 uL Aer/EnvZ, 1 uL sterile DI water.</p>
<p>5:1 - 1 uL BB, 1 uL Ligase, 1 uL Buffer, 3 uL OmpR, 3 uL Aer/EnvZ, 1 uL sterile DI water.</p>
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<p>Room Temperature for 2 hours.</p>
<p>Room Temperature for 2 hours.</p>
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<p>We received the strains with OmpR and EnvZ individually knocked out. Brandon grew these up in liquid media and put the plates in the 4 C room. We are still waiting to grow up &Delta;OmpR + &Delta;EnvZ strains.</p>
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<p>We received the strains with OmpR and EnvZ individually knocked out. Brandon grew these up in liquid media and put the plates in the 4 C room. We are still waiting to grow up ΔOmpR + ΔEnvZ strains.</p>
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<p>[<a href="https://2013.igem.org/Team:UTK-Knoxville" class="external text" rel="nofollow">back</a>]</p>
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Revision as of 05:15, 30 July 2013

Team:UK-Knoxville - 2013.igem.org

NotebookNAVI NotebookRETURN BACK NEXT

June 06rd 2013

Ivan, Brandon, Eric, and Patrick met to discuss the RcoM, HemAT, and cytoplasmic primers and the wiki page.

Ligation Aer/EnvZ + OmpR + BB

3:1 - 1 uL BB, 1 uL Ligase, 1 uL Buffer, 1.75 uL OmpR, 1.75 uL Aer/EnvZ, 3.5 uL sterile DI water.

5:1 - 1 uL BB, 1 uL Ligase, 1 uL Buffer, 3 uL OmpR, 3 uL Aer/EnvZ, 1 uL sterile DI water.

Room Temperature for 2 hours.

We received the strains with OmpR and EnvZ individually knocked out. Brandon grew these up in liquid media and put the plates in the 4 C room. We are still waiting to grow up ΔOmpR + ΔEnvZ strains.

[back]

UTK.edu UTK CBE UTK CBE Trinh Lab

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