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Team:Evry/Protocols/04
From 2013.igem.org
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<h2> LB and LB Agar </h2> | <h2> LB and LB Agar </h2> | ||
| - | Mix 20 g of LB Broth in 1L of distilled water in a bottle of 1L | + | Mix 20 g of LB Broth in 1L of distilled water in a bottle of 1L, then put in autoclave. <br> |
| - | Mix 17,5 g of LB Agar and in 500 mL of distilled water in a bottle of 1L | + | Mix 17,5 g of LB Agar and in 500 mL of distilled water in a bottle of 1L, then put in autoclave. |
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<h2> Tris HCl </h2> | <h2> Tris HCl </h2> | ||
| + | Disolve 4,6g of Trisbase in 30 mL of distilled water.<br> | ||
| + | Adjust pH with concentrated HCl(~4 mL) until pH=7,5.<br> | ||
| + | Add distilled water until 50 mL then put in autoclave.<br> | ||
| + | If the solution has a yellow coloration, do the preparation again with better Trisbase. | ||
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Revision as of 11:43, 31 July 2013
Solutions, Media and Petri Dishes
Antibiotics
LB and LB Agar
Mix 20 g of LB Broth in 1L of distilled water in a bottle of 1L, then put in autoclave.Mix 17,5 g of LB Agar and in 500 mL of distilled water in a bottle of 1L, then put in autoclave.
CaCl2 and CaCl2+glycerol
Tris HCl
Disolve 4,6g of Trisbase in 30 mL of distilled water.Adjust pH with concentrated HCl(~4 mL) until pH=7,5.
Add distilled water until 50 mL then put in autoclave.
If the solution has a yellow coloration, do the preparation again with better Trisbase.
