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Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period3/Dailylog
From 2013.igem.org
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| - | - | + | - Started E coli liquid culture over night |
| - | + | * 10mL of BL21 for spot test in [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.29 Mutagen Concentration Test - Sixth Protocol|7.29 Mutagen Concentration Test - Sixth Protocol]]. | |
| - | + | * 2mL each of BL21, W3110, and B for phage viability and infection test in [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - Experiment 1]]. | |
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Revision as of 14:40, 5 August 2013
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8/1/13 - Performed the spot test in 7.29 Mutagen Concentration Test - Sixth Protocol.
8/2/13 - Because the top agar used in yesterday's spot was contaminated, we decided to redo this step. Also, because most of the spot tests went down to -6, we performed further dilution for the next testing to get a more accurate idea of phage concentration. - Discussed modeling options: model phage plaque size against various variables including phage particle size, agar concentration, and bacterial concentration. - Streaked E coli W3110 and K12 from frozen stock in preparation for the viability test in 8.2 Modeling Phage Plaque Sizes - Experiment 1.
8/3/13 - E coli W3110 streak worked, but K12 did not survive. Thus, we tried to amplify K12 using liquid culture. For specifics, please consult 8.2 Modeling Phage Plaque Sizes - Experiment 1.
8/4/13 - Started E coli liquid culture over night
7/26/13 - Designed protocols for 7.29 Mutagen Concentration Test - Sixth Protocol and decided to conduct an experiment to verify bacteria growth curve (specifics see 7.27 Bacteria Growth Curve After Dilution) - Started 3mL of BL21 liquid culture overnight.
7/27/13 - Conducted 7.27 Bacteria Growth Curve After Dilution
7/28/13 - Started 15mL of BL21 liquid culture overnight. - Started phage liquid culture to test for superinfection.
7/29/13 - Performed part 1 of 7.29 Mutagen Concentration Test - Sixth Protocol. - Did a spot test to determine the concentration phage in yesterday's liquid culture (We couldn't test for superinfection because all the bacteria were lysed.)
7/31/13 - Started 8mL BL21 liquid culture overnight and performed dilution series for the spot test procedure in 7.29 Mutagen Concentration Test - Sixth Protocol.
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