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Team:MSOE Milwaukee/Week10
From 2013.igem.org
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<H1 align = left>Tuesday</H1> | <H1 align = left>Tuesday</H1> | ||
| - | <p>We began the procedure for making competent cells of the BL21 type. We also found a Gibson Assembly kit that we are | + | <p>We began the procedure for making competent cells of the BL21 type. We also found a Gibson Assembly kit that we are ordering to assemble our gene blocks. The kit also contains super competent cells, which will be very useful in our future protocols. It is set to arrive tomorrow. When it does, we have to get the competent cells in the -80 as soon as we can. Hopefully this will help us with our transformation efficiency in later steps of our project. We also worked on some housekeeping activities, like updating our website, the safety information, and organizing our current materials. </p><br> |
<H1 align = left>Wednesday</H1> | <H1 align = left>Wednesday</H1> | ||
Revision as of 19:55, 6 August 2013
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Week 10
Monday
As a team, we fixed a few of the protocols to add positive controls and more replicates at different concentrations for the xylose and eucalyptol procedures. We also recieved our order of xylose, eucalyptol, and our gene block parts. However, we discovered that we didn't have the Gibson Assembly master mix, which we will have to look into purchasing.
Tuesday
We began the procedure for making competent cells of the BL21 type. We also found a Gibson Assembly kit that we are ordering to assemble our gene blocks. The kit also contains super competent cells, which will be very useful in our future protocols. It is set to arrive tomorrow. When it does, we have to get the competent cells in the -80 as soon as we can. Hopefully this will help us with our transformation efficiency in later steps of our project. We also worked on some housekeeping activities, like updating our website, the safety information, and organizing our current materials.
Wednesday
Thursday
Friday
