Small Phage
From 2013.igem.org
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==May== | ==May== | ||
+ | ===5/1/13=== | ||
+ | |||
+ | LP, XL | ||
+ | |||
+ | - Commencement of spring!!! | ||
+ | - Discussed goals and outlined plans for spring term | ||
+ | |||
+ | ===5/2/13=== | ||
+ | |||
+ | LP, XL | ||
+ | |||
+ | - Designed primers for amplifying and sequencing phage capsid protein | ||
+ | |||
+ | - Wrote emails inquiring mutagen (NG) and plasmid for in vitro assembly | ||
+ | |||
+ | ===5/3/13=== | ||
+ | |||
+ | LP, XL | ||
+ | |||
+ | - Performed agar test, focusing primarily on ×8 | ||
+ | |||
+ | : [[5.3 T7 phage selection method test]] | ||
+ | |||
+ | - Processed phage amplification | ||
+ | |||
+ | : [[5.3 T7 phage amplification/purification]] | ||
+ | |||
+ | LP | ||
+ | |||
+ | - Started two 5mL overnights of BL21 | ||
===5/20/13=== | ===5/20/13=== |
Revision as of 18:19, 21 May 2013
Contents |
March
April
May
5/1/13
LP, XL
- Commencement of spring!!! - Discussed goals and outlined plans for spring term
5/2/13
LP, XL
- Designed primers for amplifying and sequencing phage capsid protein
- Wrote emails inquiring mutagen (NG) and plasmid for in vitro assembly
5/3/13
LP, XL
- Performed agar test, focusing primarily on ×8
- Processed phage amplification
LP
- Started two 5mL overnights of BL21
5/20/13
LP, XL
- We performed T7 Mutagen Concentration Test
- We performed T7 Minor Capsid Protein PCR
June
July
August
September