"
Small Phage
From 2013.igem.org
| Line 11: | Line 11: | ||
[[Winter]] | [[Winter]] | ||
| - | [[Spring]] | + | [[Team:BYU Provo/Notebook/SmallPhage/Springexp|Spring]] |
Summer | Summer | ||
| Line 20: | Line 20: | ||
'''add overview''' | '''add overview''' | ||
| - | [[ | + | Pages that needs deleting |
| + | |||
| + | [[Spring]] | ||
| style="width: 20%; background-color: transparent;"| | | style="width: 20%; background-color: transparent;"| | ||
Revision as of 13:32, 22 May 2013
| Small Phage (Add formatting) | ||
|
Overview Summer Fall |
add overview Pages that needs deleting |
add picture |
Contents |
March
April
May
5/1/13
- Commencement of spring!!!
- Discussed goals and outlined plans for spring term
5/2/13
- Designed primers for amplifying and sequencing phage capsid protein
- Wrote emails inquiring mutagen (NG) and plasmid for in vitro assembly
5/3/13
- Performed agar test, focusing primarily on ×8
- Processed phage amplification
5/4/13
- Performed dilution series using stock 5.3 (-1 through -11)
- Started two 5mL overnights of BL21
5/5/13
- Spot test using stock 5.3 and its dilution series (from 5.4)
- Started liquid culture for purification team (at around noon)
- 1mL of BL21 overnight + 4mL of LB + 100μL -2 phage (received stock)
- Started 5.5 amplification from a plaque test
5/6/13
- Continued 5.3 T7 phage amplification/purification
- Performed liquid culture phage concentration test
5/7/13
- Started two 5mL of E coli BL21 overnight
- Designed procedure for applying mutagen and selecting for T7
5/8/13
- Went over procedure for applying mutagen and PCR with Dr. Grose
- Performed spot tests under 5.6 T7+ Liquid Culture Phage Concentration Test
- Started two 5mL BL21 overnights
- Learned how to create LB plates
5/9/13
- Practiced with ×6 and ×8 top agar
- Started 5.9 T7+ Liquid Culture Phage Concentration Test #2
- Discussed plans and schedule for next week.
5/20/13
LP, XL
- We performed T7 Mutagen Concentration Test
- We performed T7 Minor Capsid Protein PCR
June
July
August
September
