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Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period3/Dailylog
From 2013.igem.org
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- Discussed modeling options: model phage plaque size against various variables including phage particle size, agar concentration, and bacterial concentration. | - Discussed modeling options: model phage plaque size against various variables including phage particle size, agar concentration, and bacterial concentration. | ||
| - | - Streaked E coli W3110 and K12 from frozen stock in preparation for the viability test in [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - | + | - Streaked E coli W3110 and K12 from frozen stock in preparation for the viability test in [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - Preliminary Experiments]]. |
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<font size="4"> '''8/3/13''' </font> | <font size="4"> '''8/3/13''' </font> | ||
| - | - E coli W3110 streak worked, but K12 did not survive. Thus, we tried to amplify K12 using liquid culture. For specifics, please consult [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - | + | - E coli W3110 streak worked, but K12 did not survive. Thus, we tried to amplify K12 using liquid culture. For specifics, please consult [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - Preliminary Experiments]]. |
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- Started E coli liquid culture over night | - Started E coli liquid culture over night | ||
* 10mL of BL21 for spot test in [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.29 Mutagen Concentration Test - Sixth Protocol|7.29 Mutagen Concentration Test - Sixth Protocol]]. | * 10mL of BL21 for spot test in [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.29 Mutagen Concentration Test - Sixth Protocol|7.29 Mutagen Concentration Test - Sixth Protocol]]. | ||
| - | * 2mL each of BL21, W3110, and B for phage viability and infection test in [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - | + | * 2mL each of BL21, W3110, and B for phage viability and infection test in [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - Preliminary Experiments]]. |
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<font size="4"> '''8/5/13''' </font> | <font size="4"> '''8/5/13''' </font> | ||
| - | - Performed Phage viability/infection test for [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - | + | - Performed Phage viability/infection test for [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - Preliminary Experiments]]. |
- Discussed ideas for modeling phage plaque sizes | - Discussed ideas for modeling phage plaque sizes | ||
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<font size="4"> '''8/6/13''' </font> | <font size="4"> '''8/6/13''' </font> | ||
| - | - Check up on the phage viability/infection test for [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - | + | - Check up on the phage viability/infection test for [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - Preliminary Experiments]]. |
- Started 5mL of E coli B liquid culture overnight. | - Started 5mL of E coli B liquid culture overnight. | ||
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<font size="4"> '''8/7/13''' </font> | <font size="4"> '''8/7/13''' </font> | ||
| - | - Performed phage titer determination for [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - | + | - Performed phage titer determination for [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - Preliminary Experiments]]. |
- Phage Purification Group started the purification process with CsCl gradient. | - Phage Purification Group started the purification process with CsCl gradient. | ||
Revision as of 22:36, 10 August 2013
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8/1/13 - Performed the spot test in 7.29 Mutagen Concentration Test - Sixth Protocol.
8/2/13 - Because the top agar used in yesterday's spot was contaminated, we decided to redo this step. Also, because most of the spot tests went down to -6, we performed further dilution for the next testing to get a more accurate idea of phage concentration. - Discussed modeling options: model phage plaque size against various variables including phage particle size, agar concentration, and bacterial concentration. - Streaked E coli W3110 and K12 from frozen stock in preparation for the viability test in 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments.
8/3/13 - E coli W3110 streak worked, but K12 did not survive. Thus, we tried to amplify K12 using liquid culture. For specifics, please consult 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments.
8/4/13 - Started E coli liquid culture over night
8/5/13 - Performed Phage viability/infection test for 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments. - Discussed ideas for modeling phage plaque sizes
8/6/13 - Check up on the phage viability/infection test for 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments. - Started 5mL of E coli B liquid culture overnight.
8/7/13 - Performed phage titer determination for 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments. - Phage Purification Group started the purification process with CsCl gradient.
8/8/13 - Started approximately 15mL of E coli B liquid culture overnight.
7/31/13 - Started 8mL BL21 liquid culture overnight and performed dilution series for the spot test procedure in 7.29 Mutagen Concentration Test - Sixth Protocol.
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