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12/08/13
From 2013.igem.org
(Difference between revisions)
(Created page with "==Sonicating herring sperm DNA== *Pipetting out sonicated samples every 30mins **Pipette 4ul of DNA into Son 1-6 every 30mins ***Dilute 1ul from Son 1-6 in 9ul of 1xTE buffer to ...") |
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*Pipetting out sonicated samples every 30mins | *Pipetting out sonicated samples every 30mins | ||
**Pipette 4ul of DNA into Son 1-6 every 30mins | **Pipette 4ul of DNA into Son 1-6 every 30mins | ||
| - | ***Dilute 1ul from Son 1-6 in 9ul of 1xTE buffer to tubes 10xdil 1-6 | + | ***Dilute 1ul of DNA from Son 1-6 in 9ul of 1xTE buffer to tubes 10xdil 1-6 |
***Vortex each dilution thoroughly and centrifuge briefly. | ***Vortex each dilution thoroughly and centrifuge briefly. | ||
*Keep tubes on ice | *Keep tubes on ice | ||
Revision as of 11:49, 12 August 2013
Sonicating herring sperm DNA
- Pipetting out sonicated samples every 30mins
- Pipette 4ul of DNA into Son 1-6 every 30mins
- Dilute 1ul of DNA from Son 1-6 in 9ul of 1xTE buffer to tubes 10xdil 1-6
- Vortex each dilution thoroughly and centrifuge briefly.
- Pipette 4ul of DNA into Son 1-6 every 30mins
- Keep tubes on ice
Running an agarose gel for sonication analysis
- To tubes 10xdil 1-6, add 5ul of water and 5ul of orange G dye. Centrifuge. Keep on ice until loading.
- Use 5ul of 1kb marker on one end, and 5ul of 100kb marker on other end.
- Run at 100V for an hour
