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12/08/13
From 2013.igem.org
(Difference between revisions)
(→Running an agarose gel for sonication analysis) |
(→Running an agarose gel for sonication analysis) |
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*To tubes 10xdil 1-6, add 5ul of sterile water and 5ul of orange G dye. Centrifuge briefly. Keep on ice until loading. | *To tubes 10xdil 1-6, add 5ul of sterile water and 5ul of orange G dye. Centrifuge briefly. Keep on ice until loading. | ||
*Use 5ul of 1kb marker on one end, and 5ul of 100kb marker on other end. | *Use 5ul of 1kb marker on one end, and 5ul of 100kb marker on other end. | ||
| + | *Add buffer so that wells in the tray and the tray itself are covered | ||
*Run at 100V for an hour | *Run at 100V for an hour | ||
Revision as of 12:01, 12 August 2013
Sonicating herring sperm DNA
- Pipetting out sonicated samples every 30mins
- Pipette 4ul of DNA into Son 1-6 every 30mins
- Dilute 1ul of DNA from Son 1-6 in 9ul of 1xTE buffer to tubes 10xdil 1-6
- Vortex each dilution thoroughly and centrifuge briefly.
- Pipette 4ul of DNA into Son 1-6 every 30mins
- Keep tubes on ice
Running an agarose gel for sonication analysis
- To tubes 10xdil 1-6, add 5ul of sterile water and 5ul of orange G dye. Centrifuge briefly. Keep on ice until loading.
- Use 5ul of 1kb marker on one end, and 5ul of 100kb marker on other end.
- Add buffer so that wells in the tray and the tray itself are covered
- Run at 100V for an hour
