Team:BYU Provo/Notebook/CholeraDetection/Springexp/Period5/Dailylog

6/24/13

KK, KP, CH

Unfortunately over the weekend our PCR gel of the 8 transformed colonies did not show that CRO had been ligated into the plasmid. Each lane on the gel showed a band that was the same as the control. It could be that the ligation failed, or perhaps we didn't choose any colony that had taken up the plasmid and CRO. Whatever the reason be, we're troubleshooting. We submitted sequencing for two of the colonies (two for which we had overnights already prepared), and then we shifted gears from Lambda and CRO to our Quorum Sensing construct, about which Clarice knows the most. It's quite confusing. The only thing I feel confident saying is that the construct is NOT what we expect it to be. It is not what last year's iGEM group says it is. Kelton and Clarice submitted many sections of the plasmid for sequencing again. I spent most of my time working on the Children's Book. Our presentation at the Orem Library is July 9th.

8/12/13

KK, KP Today we examined the results of our latest test to understand the effects of cro, amp, and arabinose on the lysogeny of lambda. We made 21 plates with different variations in the presence of cro, amp, or arabinose. We performed this test because after adding cro, we no longer see the formation of plaques. We are unsure about what is causing the lack of plaques. Unfortunately, our test yielded inconclusive data due to the fact that none of the plates had plaques. So, we are going back to the beginning to see if we lose lambda during one of the steps leading up to induction. One of our hypothesis is that something goes wrong during the electroporation process. Our plan is to do intermediate tests during the electroporation process to discover which step if any is causing the lack of plaques. We have been working for the last month and a half on trying to control the lysis of lambda using cro. As of now, we have arrived at no conclusion.

6/28/13

KK, KP

7/1/13 KK, KP,

7/3/13

KK, KP

7/5/13

KK, KP