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| + | ==Incubate in broth== | ||
| + | |||
| + | *4ml of luria broth in 6 universals | ||
| + | *Add 8ul of chloramphenicol | ||
| + | *Pick single colony and put in broth (sterile technique) | ||
| + | *Incubate overnight at 37 in shaker | ||
| + | |||
| + | |||
| + | ==Make agarose gel== | ||
| + | |||
| + | *20ml of 5xTBE | ||
| + | *80ml of water | ||
| + | *0.8g of agarose | ||
| + | (0.8% gel) | ||
| + | *boil till all agarose dissolved | ||
| + | *Cool | ||
| + | *Add 5ul of ethidium bromide | ||
| + | *Pour gel | ||
| + | |||
| + | |||
| + | ==Make buffer== | ||
| + | |||
| + | *50ml of 5xTBE | ||
| + | *200ml of water | ||
Latest revision as of 10:38, 14 August 2013
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Incubate in broth
- 4ml of luria broth in 6 universals
- Add 8ul of chloramphenicol
- Pick single colony and put in broth (sterile technique)
- Incubate overnight at 37 in shaker
Make agarose gel
- 20ml of 5xTBE
- 80ml of water
- 0.8g of agarose
(0.8% gel)
- boil till all agarose dissolved
- Cool
- Add 5ul of ethidium bromide
- Pour gel
Make buffer
- 50ml of 5xTBE
- 200ml of water
