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Exeter/7 August 2013
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GeorgiaDrew (Talk | contribs) (Created page with " Glycerol stocks *200ul of glycerol and 200ul of our overnight liquid cultures into labelled eppendorfs. *Store at -80 degrees celsius *Stocks made of: NEB1, Promotor + RBS, NEB...") |
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Glycerol stocks | Glycerol stocks | ||
*200ul of glycerol and 200ul of our overnight liquid cultures into labelled eppendorfs. | *200ul of glycerol and 200ul of our overnight liquid cultures into labelled eppendorfs. | ||
*Store at -80 degrees celsius | *Store at -80 degrees celsius | ||
*Stocks made of: NEB1, Promotor + RBS, NEB4, Cyan, RBS+Cph8, Magenta, B0015, B0034, RFP control, NEB3, Yellow | *Stocks made of: NEB1, Promotor + RBS, NEB4, Cyan, RBS+Cph8, Magenta, B0015, B0034, RFP control, NEB3, Yellow | ||
| + | |||
| + | We miniprepped: | ||
| + | |||
| + | |||
| + | * B0034 (RBS) AMP | ||
| + | |||
| + | * K592010 (yellow) CAM | ||
| + | |||
| + | * S05058 (RBS + yellow) CAM | ||
| + | |||
| + | * K608002 (Promoter and RBS) CAM | ||
| + | |||
| + | * RFP control CAM | ||
| + | |||
| + | * 3 NEB (RFP and Terminator) AMP | ||
| + | |||
| + | * K592012 (Magenta) CAM | ||
| + | |||
| + | * NEB4 (RBS and cph8) AMP | ||
| + | |||
| + | * NEB1 (RBS and cph8) AMP | ||
| + | |||
| + | * B0015 (Terminator) CAM | ||
| + | |||
| + | * K592018 (RBS + Cyan) CAM | ||
| + | |||
| + | |||
| + | == Adam and Tom == | ||
| + | |||
| + | |||
| + | Digested ligations from Tuesday out of plasmid and ran on a gel ( but used the wrong ladder! Hyper Ladder 2 and ran off the end of the gel) | ||
Revision as of 09:20, 15 August 2013
Glycerol stocks
- 200ul of glycerol and 200ul of our overnight liquid cultures into labelled eppendorfs.
- Store at -80 degrees celsius
- Stocks made of: NEB1, Promotor + RBS, NEB4, Cyan, RBS+Cph8, Magenta, B0015, B0034, RFP control, NEB3, Yellow
We miniprepped:
- B0034 (RBS) AMP
- K592010 (yellow) CAM
- S05058 (RBS + yellow) CAM
- K608002 (Promoter and RBS) CAM
- RFP control CAM
- 3 NEB (RFP and Terminator) AMP
- K592012 (Magenta) CAM
- NEB4 (RBS and cph8) AMP
- NEB1 (RBS and cph8) AMP
- B0015 (Terminator) CAM
- K592018 (RBS + Cyan) CAM
Adam and Tom
Digested ligations from Tuesday out of plasmid and ran on a gel ( but used the wrong ladder! Hyper Ladder 2 and ran off the end of the gel)
