Team:BYU Provo/Notebook/SmallPhage/Springexp/Period2/Exp/5.13 Determining E coli concentration with spectrophotometer
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'''I) Purpose''' | '''I) Purpose''' | ||
: To determine if OD600 can be used to determine E coli concentration | : To determine if OD600 can be used to determine E coli concentration | ||
- | : | + | : According to reference The number of cells in a bacterial culture can be estimated by reading the absorbance at 600nm (OD600). For E. coli cells, an OD600 of 1.0 ≈ 5 x |
+ | 108 cells/ml | ||
'''II) Expected Outcome''' | '''II) Expected Outcome''' |
Revision as of 21:41, 27 May 2013
Small Phage Spring Notebook: Experiments
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5.13 Determining E coli Concentration With Spectrophotometer
I) Purpose
108 cells/ml II) Expected Outcome
III) Reagants Used
1. Dilution series
2. Measurements using spectrophotometer
1. Raw data
2. Plot ADD 3. Linear Regression Result ADD VI) Conclusion
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