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16/08/13
From 2013.igem.org
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(Created page with "==Running the gel from sonication series== *Samples were taken from sonicated tube every 30min intervals, a total of 6 samples. *Each was diluted 100 fold to get a concentration ...")
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(Created page with "==Running the gel from sonication series== *Samples were taken from sonicated tube every 30min intervals, a total of 6 samples. *Each was diluted 100 fold to get a concentration ...")
Newer edit →
Revision as of 13:39, 16 August 2013
Running the gel from sonication series
- Samples were taken from sonicated tube every 30min intervals, a total of 6 samples.
- Each was diluted 100 fold to get a concentration in the range of 40-50ng/ul
- 5ul of DNA was taken from the dilution, to which 10ul of sterile water and 5ul of orange G was added
- Samples were run against a 1kb ladder and control herring sperm DNA, which was not sonicated
