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Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period3/Explist
From 2013.igem.org
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| - | <font size="4"> '''8.2 Modeling Phage Plaque Sizes - Preliminary | + | <font size="4"> '''8.2 Modeling Phage Plaque Sizes - Preliminary Experiments''' </font> |
We performed this experiment to establish the phage stocks we can use for our modeling project. With this experiment, we were also able to determine the concentration to phage we need to use and several variable we have to control for in our actual experiments. | We performed this experiment to establish the phage stocks we can use for our modeling project. With this experiment, we were also able to determine the concentration to phage we need to use and several variable we have to control for in our actual experiments. | ||
| - | Detailed procedure: [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - Preliminary | + | Detailed procedure: [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - Preliminary Experiments]]. |
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<font size="4"> '''8.14 Mutagen Concentration Test - Seventh Protocol''' </font> | <font size="4"> '''8.14 Mutagen Concentration Test - Seventh Protocol''' </font> | ||
| - | This is our seventh round of mutagenesis. We were able to finalize our protocol for T7 mutagenesis with [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.29 Mutagen Concentration Test - Sixth Protocol|7.29 Mutagen Concentration Test - Sixth Protocol]]. However, the Phage Purification Team needed more sample to finalize their CsCl protocol. Thus, we performed this new round of mutagenesis. We also switched to E coli B after establishing in [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - Preliminary | + | This is our seventh round of mutagenesis. We were able to finalize our protocol for T7 mutagenesis with [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.29 Mutagen Concentration Test - Sixth Protocol|7.29 Mutagen Concentration Test - Sixth Protocol]]. However, the Phage Purification Team needed more sample to finalize their CsCl protocol. Thus, we performed this new round of mutagenesis. We also switched to E coli B after establishing in [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - Preliminary Experiments]] that E coli B also support the growth of T7. |
Detailed procedure: [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.14 Mutagen Concentration Test - Seventh Protocol|8.14 Mutagen Concentration Test - Seventh Protocol]]. | Detailed procedure: [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.14 Mutagen Concentration Test - Seventh Protocol|8.14 Mutagen Concentration Test - Seventh Protocol]]. | ||
Revision as of 22:00, 17 August 2013
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8.2 Modeling Phage Plaque Sizes - Preliminary Experiments We performed this experiment to establish the phage stocks we can use for our modeling project. With this experiment, we were also able to determine the concentration to phage we need to use and several variable we have to control for in our actual experiments. Detailed procedure: 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments.
8.14 Mutagen Concentration Test - Seventh Protocol This is our seventh round of mutagenesis. We were able to finalize our protocol for T7 mutagenesis with 7.29 Mutagen Concentration Test - Sixth Protocol. However, the Phage Purification Team needed more sample to finalize their CsCl protocol. Thus, we performed this new round of mutagenesis. We also switched to E coli B after establishing in 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments that E coli B also support the growth of T7. Detailed procedure: 8.14 Mutagen Concentration Test - Seventh Protocol.
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