19/08/13
From 2013.igem.org
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(Created page with "==Denaturing sonicated DNA== *Denaturing DNA by heat shock for 10mins in a 95C heat block *Adding 5ul of OG dye ==Running both denatured and sonicated samples on gel== *For just...")
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(Created page with "==Denaturing sonicated DNA== *Denaturing DNA by heat shock for 10mins in a 95C heat block *Adding 5ul of OG dye ==Running both denatured and sonicated samples on gel== *For just...")
Newer edit →
Revision as of 11:04, 19 August 2013
Denaturing sonicated DNA
- Denaturing DNA by heat shock for 10mins in a 95C heat block
- Adding 5ul of OG dye
Running both denatured and sonicated samples on gel
- For just the sonication samples 1-6, 5ul of OG and 10ul of sterile water was added to 5ul of DNA.