20/08/13

From 2013.igem.org

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(→Plating out bacteria)
 
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!align="center"|[[Team:Leicester|Home]]
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Leicester Official Team Profile]
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!align="center"|[[Team:Leicester/Project|Project]]
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!align="center"|[[Team:Leicester/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Leicester/Modeling|Modeling]]
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!align="center"|[[Team:Leicester/Notebook|Notebook]]
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!align="center"|[[Team:Leicester/Safety|Safety]]
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!align="center"|[[Team:Leicester/Attributions|Attributions]]
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==Making nutrient agar plates and broth==
==Making nutrient agar plates and broth==
*Making up broth and agar solution to grow ''P. putida'' in.
*Making up broth and agar solution to grow ''P. putida'' in.

Latest revision as of 09:18, 22 August 2013

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions



Making nutrient agar plates and broth

  • Making up broth and agar solution to grow P. putida in.
  • For agar plates:
    • 9.2g of nutrient agar medium
    • 400ml of distilled water
  • For broth:
    • 3.2g of nutrient broth
    • 400ml of distilled water
  • Both were autoclaved for 45mins
  • Agar was poured onto 16 plates

Plating out bacteria

  • Freeze dried P. putida was rehydrated using 1ml of nutrient broth
  • The culture was aseptically transferred back into the broth containing tube
  • Mix
  • From that tube, colonies were streaked onto 6 plates and left to incubate over night at 30C

Growing bacteria in broth

  • 100ml of autoclaved broth was added to 2 flasks each
  • The broth tube, containing the bacteria, was divided between flasks
  • Flask were left to incubate at 30C on a shaker overnight
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