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Team:BYU Provo/Notebook/CholeraDetection/Springexp/Period3/Dailylog
From 2013.igem.org
| Cholera Detection May - June Notebook: May 27 - June 9 Daily Log
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5/28/13 - Started three 8mL E coli BL21 liquid culture at around 4pm.
5/29/13 - Continued 5.20 Mutagen Concentration Experiment - Prepared sample for sequencing. This is done as part of 5.20 T7 Minor Capsid Protein PCR - Dr. Studier responded to our email today! We also received the E coli stains containing plasmids with clone T7 genes. WE CAN START SITE DIRECTED MUTAGENESIS SOON!
5/30/13 - Plates from yesterday are taken out of incubation at around 4:00pm
5/31/13 - Discussed results for 5.20 Mutagen Concentration Experiment -> will need to try this one more time with minor adjustment to experimental design: plate -4 dilution using x6 and x8 top agar. - Discussed plans for next week. - Made new LB and x6 top agar.
6/2/13 - Made about 20ml of BL21 overnight
6/3/13 - Made new LB plates - Plated -4 titer on x6 and x8 plates for 5.20 Mutagen Concentration Experiment
6/5/13 Regarding our PCR attempt yesterday, we did see a thick fat band ... at the bottom of the gel! Once again our PCR failed. This has led us to propose several reasons why we can't PCR amplify CRO: 1) the Lambda prophage isn't actually in TT 9901 or TT9907, 2)
5/20/13 - Performed T7 Mutagen Concentration Test - Performed T7 Minor Capsid Protein PCR
5/21/13 - Started two 5mL E coli BL21 overnight at around 7:00pm
5/22/13 - Performed spot test for 5.20 Mutagen Concentration Experiment - Ran agarose gel to confirm PCR product
5/23/13 - Started two 25mL E coli BL21 liquid culture over night at around 6:00pm
5/24/13 - Proceeded with 5.20 Mutagen Concentration Experiment by performing preliminary selection using x8 top agar
5/25/13 - Took pictures in preparation for Progress Report
5/31/13 - Worked on transferring our notebook over to the iGEM wiki.
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