L-forms

Purposes

To create a BioBrick that enables the conversion of cell-walled B. subtilis cells into L-form cells without their cell walls, and back to walled cells.

Aims

• Design a biobrick which places murE under the control of a controllable promoter.

• Have this designed biobrick synthesised.

• Integrate the designed biobrick into B. subtilis.

• Determine the functionality of the Biobrick in removing the cell wall.