Team:UNITN-Trento/Parts

Revision as of 21:29, 20 September 2013 by Ggirelli (Talk | contribs)

Ethylene

	BBa_K1065000	EFE
	Ethylene Forming Enzyme gene (EFE) that forms ethylene starting from 2-oxoglutarate.

	BBa_K1065002	EFE with double-terminator
	EFE followed by the double terminator BBa_B0015.

	BBa_K1065001	EFE arabinose-regulated
	This is EFE followed by a double terminator (B0015) under the control of the araCpBAD inducible promoter.

Methyl salicylate

	BBa_K1065100	BSMT1
	This part encodes BSMT1, an enzyme that methylates salycilic acid to obtain methyl salycilate. It is the original part from the 'Eau de coli' MIT iGEM 2006 project, re-submitted (and resurrected) by us in pSB1C3.

	BBa_K1065103	PchBA
	PchA is the enzyme responsible for the formation of isochorismate from chorismate. The next step is performed by the PchB enzyme, which converts isochorismate in salicylic acid.

	BBa_K1065104	SAM synthetase
	This part encodes the SAM (S-AdoMet) synthetase gene, which we friendly call "uncle SAM". SAM is a methyl donor required to produce MeSA and other methylated molecules.

	BBa_K1065101	BSMT1 arabinose-regulated
	inducible A methyltransferase (BSMT1) regulated by the araCpBAD promoter.

	Bba_K1065105	SAM synthetase under pLac promoter
	This is SAM synthetase (uncle SAM) regulated by the costitutive Lac promoter.

	BBa_K1065102	PchBA IPTG-regulated and BSMT1 arabinose-regulated
	This is EFE followed by a double terminator (B0015) under the control of the araCpBAD inducible promoter.

	BBa_K1065106	BSMT1, PchBA and SAM synthetase
	An improved version of the wintergreen device with BMST1, PCHBA (BBa_K1065102) with added SAM synthetase enzymes to boost wintergreen production.

Blue light

	Bba_K1065301	amilCP IPTG-regulated
	We attached pLac promoter to amilCP gene. This part does not work (sadly we saw no blue color). In fact, we started from BBa_K592009 which lacks the RBS (it is a coding part). No RBS, no party!

	Bba_K1065300	BBa_K952003 with Yf1 and FixJ IPTG-regulated.
	pLac+Yf1-FixJ-pFixK2-amil GFP without RBS and with pLac upstream.

	BBa_K1065303	Yf1+FixJ+pFixK2
	The starting point to build a blue-light sensing circuit: the photosensor, the response regulator with its promoter. This part was build merging BBa_K592016 and BBa_K592006.

	BBa_K1065310	Yf1+FixJ+pFixK2+Inverter+pLambda+amilCP
	This is a complete blue-light sensing circuit, inverted with cI. Yf1 and FixJ are under the Anderson promoter (BBa_J23100). pLambda regulates the expression of the reporter gene amilCP. When exposed to blue-light, amilCP is synthesized, when the blue-light is not present, amilCP is not produced.

	BBa_K1065302	BBa_K1065300 improved
	The same as BBa_K1065300: we performed a mutagenesis to add the missing RBS, now this works!!! It is: pLac+Yf1+FixJ+pFixK2+amilGFP.

	BBa_K1065305	BBa_K952003 improved
	The same as BBa_K952003: we performed a mutagenesis to add the missing RBS, now this works!!! It is: Yf1+FixJ+pFixK2+amilGFP.

	BBa_K1065306	J23100+Yf1+FixJ
	Yf1 and FixJ under the Anderson promoter (BBa_J23100).

	BBa_K1065204	pXyl+EFE
	EFE in pXyl vector, specific for B. subtilis.

	BBa_K1065309	BBa_K1065302+BBa_K1065002
	Complete blue-light sensing circuit without inverter, producing both amilGFP and EFE.

Bacillus subtilis

	BBa_K1065201	pXyl+GFP
	An improved version of the wintergreen device with BMST1, PCHBA (BBa_K1065102) with added SAM synthetase enzymes to boost wintergreen production.

	BBa_K1065106	BSMT1, PchBA and SAM synthetase
	A B. subtilis xylose inducible plasmid (from LMU Munich iGEM 2012) to produce GFPmut3b, regulated by a strong ribosome binding site (Bba_e0840).

	BBa_K1065204	pXyl+EFE
	EFE in pXyl vector, specific for B. subtilis.

	BBa_K1065311	Complete blue-light sensing device with inverter, amilCP and EFE
	Our complete circuit composed by:J23100, Yf1 and FixJ, pFixK2, CI, pLambda, amilCP and EFE.

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