Team:UNITN-Trento/Parts

Revision as of 08:55, 21 September 2013 by Ggirelli (Talk | contribs)

In this page you can find the fruits of our summer’s efforts. The number of bananas quantifies how much we like each part:

(1 banana): it does not work, due to a mistake of some kind, or we do not know if it works and more tests must be performed.
(2 bananas): it works as expected but it is not characterized yet.
(3 bananas): it works as expected and has been characterized.

♫ ♪ “One banana, two bananas, three bananas, four. Four bananas make a bunch and so do many more.” ♪ ♫

[cit. The Banana Splits – The Tra La La Song]

Ethylene

	BBa_K1065000	EFE
	Ethylene Forming Enzyme gene (EFE) that forms ethylene starting from 2-oxoglutarate.

	BBa_K1065002	EFE with double-terminator
	EFE followed by the double terminator BBa_B0015.

	BBa_K1065001	EFE arabinose-regulated
	This is EFE followed by a double terminator (B0015) under the control of the araCpBAD inducible promoter.

Methyl salicylate

	BBa_K1065100	BSMT1
	This part encodes BSMT1, an enzyme that methylates salycilic acid to obtain methyl salycilate. It is the original part from the 'Eau de coli' MIT iGEM 2006 project, re-submitted (and resurrected) by us in pSB1C3.

	BBa_K1065103	PchBA
	PchA is the enzyme responsible for the formation of isochorismate from chorismate. The next step is performed by the PchB enzyme, which converts isochorismate in salicylic acid.

	BBa_K1065104	SAM synthetase
	This part encodes the SAM (S-AdoMet) synthetase gene, which we friendly call "uncle SAM". SAM is a methyl donor required to produce MeSA and other methylated molecules.

	BBa_K1065101	BSMT1 arabinose-regulated
	A methyltransferase (BSMT1) regulated by the araCpBAD promoter.

	BBa_K1065105	SAM synthetase under pLac promoter
	This is SAM synthetase (uncle SAM) regulated by the constitutive Lac promoter.

	BBa_K1065102	PchBA under pLac and BSMT1 arabinose-regulated
	The wintergreen device: PchBA under a mutant Lac promoter (BBa_K1065107) followed by BSMT1 under the araCpBAD promoter.

	BBa_K1065106	BSMT1, PchBA and SAM synthetase
	An improved version of the wintergreen device: BBa_K1065102 with SAM synthetase to boost production.

Blue light

	Bba_K1065301	amilCP under pLac promoter
	We attached pLac promoter to the amilCP gene. This part does not work. In fact, we started from BBa_K592009 which lacks the RBS (it is a coding part). No RBS, no party!

	BBa_K1065306	J23100+Yf1+FixJ
	Yf1 (photosensor) and FixJ (response regulator) under the Anderson promoter (BBa_J23100).

	BBa_K1065303	Yf1+FixJ+pFixK2
	The starting point to build a blue-light sensing circuit: the photosensor (Yf1) and the response regulator (FixJ) with its promoter (pFixK2). This part was build merging BBa_K592016 and BBa_K592006.

	Bba_K1065300	BBa_K952003 with Yf1 and FixJ under pLac promoter.
	pLac+Yf1-FixJ-pFixK2-amil GFP constructed adding the pLac promoter upstream of BBa_K952003, which lacks the RBS before amil GFP.

	BBa_K1065302	BBa_K1065300 improved
	The same as BBa_K1065300: we performed a mutagenesis to add the missing RBS, now it works!!! Just to remember: pLac+Yf1+FixJ+pFixK2+amilGFP.

	BBa_K1065305	BBa_K952003 improved
	The same as BBa_K952003: we performed a mutagenesis to add the missing RBS, now this works!!! Just to remember: Yf1+FixJ+pFixK2+amilGFP.

	BBa_K1065309	BBa_K1065302+BBa_K1065002
	Complete blue-light sensing circuit without inverter, producing both amilGFP and EFE.

	BBa_K1065310	Yf1+FixJ+pFixK2+Inverter+pLambda+amilCP
	This is a complete blue-light sensing circuit, inverted with cI. Yf1 and FixJ are regulated by the Anderson promoter (BBa_J23100). pLambda regulates the expression of the reporter gene amilCP. When exposed to blue-light, amilCP is synthesized, when the blue-light is not present, amilCP is not produced.

	BBa_K1065311	Complete blue-light sensing device with inverter, amilCP and EFE
	Our complete circuit composed by:J23100, Yf1 and FixJ, pFixK2, CI, pLambda, amilCP and EFE.

Bacillus subtilis

	BBa_K1065201	pXyl+GFP
	A B. subtilis xylose inducible plasmid (from LMU Munich iGEM 2012) to produce GFPmut3b, regulated by a strong ribosome binding site (Bba_e0840).

	BBa_K1065203	Efe+Bba_B0015 in pSpac.
	EFE in pSpac vector, specific for B. subtilis.

	BBa_K1065204	pXyl+EFE
	EFE in pXyl vector, specific for B. subtilis.

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