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Team:BYU Provo/Notebook/Cholera - Detection/Springexp
From 2013.igem.org
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May 1 - May 12
We attempted to amplify Cro through pcr and cut it out of a gel. Cro will be used to induce the lytic cycle of lambda. We believe that when there is an abundance of cro, there will be an increased lysis of lambda.
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| May 13 - May 26
Moving right along, we cloned the CRO gene from bacteriophage lambda into pIG12, a vector with the arabinose-inducible pBAD promoter. We transformed the ligated plasmid into our target strains, TT9901, TT9907 (which have bacteriophage lambda integrated into their genomes as a prophage) and TT25281 (our control without the lysogen) by electroporation.
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| May 27 - June 9
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| June 10 - June 23
With the correct primers, DMSO, and fresh template, we performed a successful PCR reaction of CRO. We continued the cloning process by digesting pIG12 and CRO, ligating them together, and transforming the ligated plasmid into DH5a E.Coli.
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| June 24 - June 30
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