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Team:SUSTC-Shenzhen-A/Project
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Most people might have heard a classic model called prisoners’ dilemma .It is the inspiration of our project . The prisoners’dilemma is a special “game”between two arrested prisoners . What the story tells about is that two suspects were caught by the police and they were questioned in two separated rooms . Because of the lack of evidence , the police could not convict them of the crime. Then ,the police offered them a deal . the sentence that each receives depends both on their own decisions whether to confess or remain the silent and on the decision made by the other. This case is always used to state that the best choice for the individual is not the best choice for the whole team . Our idea is that we could import two different constructed plasmid into the Ecoli, then we could simulate the prisoners’dilemma by adding different chemicalsubstance which could regulate the expression of the gene when culturing . The growth situations of the two bacteria are the results of their “choices”. | File:IBB-Pune team.png Your team picture |
Project Details
Part 1
Part 2
The Experiments
Part 3
Results
Biobricks checking
<p>We chose four biobricks to detect after search . They are xylose、KNO3、glucose and 12HSL.xylose
KNO3
Compared with the results of the efficiency of the xylose inducible promoter detected by the HKT, our consequence differed from them in some aspects, or better. We had used the FCM to detect it. There was an obvious tendency that the efficiency of the promoter increased as the xylose concentration gathered up. What was amazing was that the fluorescence we got was 10 times brighter than the result the HKT got, which appeared to be great.
But when the concentration of the xylose was 0.03% and 0.3%, we got an unexpected result that the expression of the GFP fell after rise. What’s more. While the concentration of the xylose was 10%, the expression of the GFP decreased, which corresponded with the result of the HKT.
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