Results
1.IL10recepter
- We inserted Il10receptere gene into vecter
1 transformation
- Figure 1transformation of Il10recepter α
- Figure2 transformation of Il10recepter β
2 .culture on the LB nutrient medium and get colonies.
3 colony PCR
- The molecular weight of the α chain is 1734bp. The molecular weight of the α chain is 975bp
- Figure3 Il10recepter α colony PCR
:*Figure4 Il10recepter β colony PCR
4 restriction enzyme processing PCR production
5 infusion
6 .transformation
7 culture on the LB nutrient medium and get colonies.
8 colony PCR
- The molecular weight of the vector is a 4000 ~ 5000bp.
- We were not able to confirm the vector by colony PCR.
2 STAT3
- We inserted stat3 gene into vector
1 transformation
- Figure5 transformation of STAT3
2 .culture on the LB nutrient medium and get colonies.
3 colony PCR
- The molecular weight of the α chain is 2307bp.
4 restriction enzyme processing PCR production
5 infusion
6 .transformation
7 culture on the LB nutrient medium and get colonies.
8 colony PCR
- The molecular weight of the vector is a 4000 ~ 5000bp.
- We were not able to confirm the vector by colony PCR.
3 GFP+HlyA
- We inserted GFP gene and HlyA into vecter
1 transformation
- Figure 1transformation of GFP.
- Figure2 transformation of HlyA.
2 .culture on the LB nutrient medium and get colonies.
3 colony PCR
- The molecular weight of the α chain is 720bp. The molecular weight of the α chain is 186bp
- Figure3 Il10recepter GFP PCR
- Figure4 Il10recepter HlyA PCR
4 restriction enzyme processing PCR production
5 infusion
6 .transformation
7 culture on the LB nutrient medium and get colonies.
8 colony PCR
- The molecular weight of the vector is a 2000~3000bp.
- We were not able to confirm the vector by colony PCR.
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