Team:KAIT Japan/Results

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Results

1.IL10recepter

We inserted Il10receptere gene into vecter

1 transformation
Figure1.jpeg

  • Figure 1transformation of Il10recepter α

Figure2.jpeg

  • Figure2 transformation of Il10recepter β

2 .culture on the LB nutrient medium and get colonies.
3 colony PCR

The molecular weight of the α chain is 1734bp. The molecular weight of the α chain is 975bp

Fig12.png

  • Figure3 Il10recepter α colony PCR

Fig2.png ‎

  • Figure4 Il10recepter β colony PCR


4 restriction enzyme processing PCR production
5 infusion
6 .transformation
7 culture on the LB nutrient medium and get colonies.
8 colony PCR

The molecular weight of the vector is a 4000 ~ 5000bp.
We were not able to confirm the vector by colony PCR.


2 STAT3

We inserted stat3 gene into vector


1 transformation
Figure5.jpeg

  • Figure5 transformation of STAT3

2 .culture on the LB nutrient medium and get colonies.
3 colony PCR

The molecular weight of the α chain is 2307bp.

Fig6.png

  • Figure6 STAT3 colony PCR


4 restriction enzyme processing PCR production
5 infusion
6 .transformation
7 culture on the LB nutrient medium and get colonies.
8 colony PCR

The molecular weight of the vector is a 4000 ~ 5000bp.
We were not able to confirm the vector by colony PCR.


3 GFP+HlyA

We inserted GFP gene and HlyA into vecter


1 transformation
Figure7.jpeg

  • Figure7 transformation of HlyA.

2 .culture on the LB nutrient medium and get colonies.
3 colony PCR

The molecular weight of the α chain is 720bp. The molecular weight of the α chain is 186bp

HlyA1.jpg

  • Figure8 Il-10recepter GFP PCR

GFP.png

  • Figure9 Il-10recepter HlyA PCR


4 restriction enzyme processing PCR production
5 infusion
6 .transformation
7 culture on the LB nutrient medium and get colonies.
8 colony PCR
Li.png

The molecular weight of the vector is a 2000~3000bp.
We were not able to confirm the vector by colony PCR.






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