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The beast and its Achilles heel:
A novel target to fight multi-resistant pathogenic bacteria
Reporter Team
Introduction
In order to facilitate the development of new antibiotics, we want to build an “Antibiotic Detector” that can identify substances influencing the homeostasis of cyclic-di-AMP (c-di-AMP). c-di-AMP is an essential signal nucleotide in most Gram-positive bacteria. It is not found in humas and several Gram-negative bacteria lack this compound, as well, including E. coli. For more information see Our Project overview.
For constructing a c-di-AMP detector, we need sensors for c-di-AMP and in order to visualize the output of the sensors, we need a reporter.
DarR reporter system ([http://parts.igem.org/Part:BBa_K1045017|part BBa_K1045017]):
Reference:
1. <a href="http://www.jbc.org/content/288/5/3085" style="color:#303030" >Zhang et.al.(2013)DarR, a TetR-like Transcriptional Factor, Is a Cyclic Di-AMP-responsive Repressor in Mycobacterium smegmatis, J. Biol. Chem. 288:3085–3096</a>
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