Team:Tuebingen/Notebook/Protocols

• Home

• Project
  • Overview
  • Motivation
  • Receptor
  • Inverter
  • Reporter
  • Plasmids
  • Application
  • Collaboration
  • Safety
• Team
  • About us
  • Attributions
• Notebook
  • Protocols
  • Overview: Journal
  • Weekly-Journal
• Activities
  • SynBio-Day
  • BMBF-Conference
  • School-Project
• Results
  • Modeling
  • Overview
  • Shipped Parts

• Sponsors
• Contact us

General protocols and E. coli protocols

• SOB medium
• LB medium
• TAE buffer
• LB plates
• Gelelectrophoresis
• Chemo-competent E. coli cells
• E. coli transformation
• pGEM Ligaton - ligation for TA-cloning of PCR products
• Ligation of digested parts and vectors
• Glycerol stocks
• PCR
• 3A-Assembly
• Preparative restriction digest
• Control restriction digest
• Mini-Prep
• Gelextraction
• Colony-PCR

Saccharomyces cerevisiae specific protocols

• YPD medium
• SC DropOut
• Yeast transformation