Team:Paris Bettencourt/Parts

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Parts Submitted and Assembly Standard

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BioBricks

BackBones

We upgraded the standard biobrick shipping vector pSB1C3 with a new set of 4 ori’s and antibiotic resistance cassettes so that they can easily be co-expressed.The system of 4 backbones all have compatible origins and resistance cassettes that allows for up to 4 back bones to be expressed in a single host bacteria.

Assembly Standard

Here, we show a novel suggestion for a standard. This suggestion opens up the possibility of keeping the BioBrick standard and adds at the same time the needed tools to perform the assembly of several parts in one step. BBG is a fusion out of the BioBrick standard cloning and Gibson isothermal assembly.

Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
75014 Paris, France
+33 1 44 41 25 22/25
team2013@igem-paris.org
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