We have engineered bacteria to turn unrecyclable waste into bioplastic

We have taken an expensive byproduct of recycling facilities, a type of mixed waste and turned it into something quite amazing. A material that can be used for a diverse range of applications from making your lunchbox to a 3D printed tissue scaffold; this material is the bioplastic poly-3-hydroxybutyric acid(P3HB). Our system is designed to maximise the recovery of resources from the waste and so we have also investigated how we can use the oil based plastics within it to produce the comommodity chemical ethylene glycol. We are passionate about using synthetic biology to help us move towards a more sustainable economy. We have considered what happens to the material we produce after it is used. This led us to develop the first synthetic biology recycling system for P3HB and one of the first in the world. The other well known bioplastic, poly lactic acid (PLA) is also expanding in use and so we developed a system to turn it back into its constituent monomers so that it can also be resynthesised, chemically or in vivo.

Click here to find out how we made P3HB and broke down oil derived plastics and here to see how we recycled P3HB and PLA.

Our System: Modular and Plastic Looping E. coli (M.A.P.L.E.)

Module 1: Resource-full Waste

Non-recyclable waste is sourced from a recycling centre, placed in a bioreactor with our M.A.P.L.E system which degrades the waste and synthesises the bioplastic P(3HB).

Module 1 Headlines

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Module 2: Plastic Fantastic

Plastic Fantastic is a complete P(3HB) bioplastic recycling platform, where P(3HB) is degraded into monomeric form and then re-polymerised back into de novo P(3HB) for future applications.

Module 2 Headlines

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Real World Research Guided the development of our Project

We thoroughly investigated how the system we have created would fit into the real world. We have identified a waste material which is an economic burden to those who produce it, this material is called Solid Recovered Fuel(SRF) but we will refer to it as mixed waste from now on. By using this waste we can not only make a profit but also offer a more environmentally sound option for dealing with the waste where the alternative is polluting and resource inefficient.

The product we are producing has many intrinsic benefits such as being industrially biodegradable and produced from sustainable sources as well as being a commodity with increasing market share.

Trips to a materials recovery facility and London City Hall highlighted the fact that recovering value from waste materials is one of the primary aims of waste management in London, the UK and indeed Europe. Importantly it highlighted the need for social solutions combined with technical ones to reduce the waste we produce in the first place and then to maximise the recovery of as much of it as possible. We promoted reducing waste and recycling across campus with our posters highlighting 5 waste offenders and through a strong social media campaign highlighting shocking and interesting issues.

MAPLE- a technology to change your mind

We have thought a lot about how our technology could change into the future and the impacts it might have. From this MAPLE evolved - Modular and Plastic Looping E.coli. We pictured how the technology could be used in different settings with various implications. In picturing this we were influenced by the DIY biology culture which is springing up and the the homebrew industry. We were intrigued by the prospect of coupling local bioplastic recycling with 3D printing and how people could start to see their waste plastic as something valuable, something which could become something beautiful and useful. You can find out more here about MAPLE's different incarnations.

Communicating our Project

We have communicated our project at several events and across social media. Once we had produced a palette of different colours from various bacterial pigments, we set about recreating painting masterpieces using the humble media of E.coli on agar petri dishes. People loved these and it brought many visitors to our pages. They became so popular that people started putting in requests, with Calgary iGEM team giving us the inspiration for our bacterial 'The Jack Pine'.

The Celebration of Science was a week's long spread of science events covering all sorts of topics. We presented our project as part of a series of lectures. Our talk was very well received as something of a contrast to the classical study of biology which had preceded us. The people we spoke to afterwards were particularly interested in the difficulties of engineering biology and also the capabilities of the technology.

Collaborations

Yale: PLA-degradation

As soon as we found out Yale iGEM team was synthesizing poly lactic acid (PLA) this year, we contacted them and sent them our PLA degradation parts to test on their product. We did this to further characterise the part and see if Biobricks behave the same on both sides of the Atlantic! When Yale optimises PLA production, they will send some of their product to break down. Breaking down PLA is a second biological bioplastic recycling system we are working on, capable of producing the monomers required to chemically resynthesise it.

UCL:Beta-Amyloid Degradation

When we heard UCL's project was to degrade Beta-Amyloid plaques we were interested to see the results as degrading a substrate has a lot in common with our project. Beta-Amyloid is a component of Amyloid Plaques which are a pathological feature of Alzheimer's disease (AD), which UCL iGEM is targeting this year.It turns out that proteinase K is not only a good way to break PLA down, but also to degrade amyloid plaques. We were interested to see if our biobrick could be multi-functional so we sent UCL a copy of it. We also built a degradation model to help the UCL team with their beta-amyloid degradation assays. The model, a MATLAB based extension called Simbiology, provides information about how efficient MMP-9 enzyme can degrade beta-amyloid plaques, which is informative for wetlab work.

St. Paul's School iGEM team

From left to right: Sisi Fan (Imperial College), Iain Bower (Imperial College),Paul-Enguerrand Fady (St. Pauls), Margarita Kopniczky (Imperial College), Freddie Wilkinson (St. Pauls), and Guy Kirkpatrick (St. Pauls).

We met the team from St.Paul's School when we went to the Young Synthetic Biology event. We were impressed by their project of the previous year and got chatting to them. As school students they were keen to see some University Labs and discuss their project further so we invited them for a tour of the facilities at Imperial. We hope the tour was enjoyable and that the tips we gave them come in useful in their entry this year.

If you want to know more about our collaborations please see our collaboration page.