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15/08/13
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Revision as of 13:29, 15 August 2013 by AliceHaworth (Talk | contribs)
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Serial dilution of herring sperm DNA
- 100ul DNA and 900ul TE to make master mix
- 5 independent samples 10ul each were taken from master mix
- Each independent sample was diluted twice in 90ul of TE
- 20 dilution samples were then nanodropped to check dilution accuracy
| Sample nr | Concentration (ng/ul) | 260/280 | 260/230 |
| nr. 1/1 | 529.9 | 1.81 | 2.26 |
| nr. 1/2 | 52.1 | 1.86 | 2.32 |
| nr. 2/1 | 454.3 | 1.81 | 2.28 |
| nr. 2/2 | 46 | 1.82 | 2.23 |
| nr. 3/1 | 457.3 | 1.83 | 2.28 |
| nr. 3/2 | 45.1 | 1.85 | 2.38 |
| nr. 4/1 | 522.8 | 1.82 | 2.27 |
| nr. 4/2 | 47.3 | 1.87 | 2.26 |
| nr. 5/1 | 481.4 | 1.82 | 2.28 |
| nr. 5/2 | 40.8 | 1.84 | 2.35 |
- Dilutions show accuracy
- Master dilution is sonicated and every 30mins a 30ul sample is taken to dilute twice (10ul of DNA in 90ul of TE buffer)
- 6 30ul samples are taken
- Samples are then run on a gel
