Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period3/Dailylog

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Small Phage July - August Notebook: August 1 - August 16 Daily Log

Small Phage

8/1/13

- Performed the spot test in 7.29 Mutagen Concentration Test - Sixth Protocol.

8/2/13

- Because the top agar used in yesterday's spot was contaminated, we decided to redo this step. Also, because most of the spot tests went down to -6, we performed further dilution for the next testing to get a more accurate idea of phage concentration.

- Discussed modeling options: model phage plaque size against various variables including phage particle size, agar concentration, and bacterial concentration.

- Streaked E coli W3110 and K12 from frozen stock in preparation for the viability test in 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments.

8/3/13

- E coli W3110 streak worked, but K12 did not survive. Thus, we tried to amplify K12 using liquid culture. For specifics, please consult 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments.

8/4/13

- Started E coli liquid culture over night

10mL of BL21 for spot test in 7.29 Mutagen Concentration Test - Sixth Protocol.
2mL each of BL21, W3110, and B for phage viability and infection test in 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments.

8/5/13

- Performed Phage viability/infection test for 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments.

- Discussed ideas for modeling phage plaque sizes

8/6/13

- Check up on the phage viability/infection test for 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments.

- Started 5mL of E coli B liquid culture overnight.

8/7/13

- Performed spot test to estimate phage titerfor 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments.

- Phage Purification Group started the purification process with CsCl gradient.

8/8/13

- Started approximately 15mL of E coli B liquid culture overnight.

8/9/13

- Performed Preliminary Titer for 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments.

8/11/13

- Started approximately 20mL of E coli B liquid culture overnight.

8/12/13

- Performed titer - repeat for 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments based on the results from 8.10 preliminary titer test.

- Started T1 propagation.

- Made accurate x2 top agar as preparation for 8.16 Modeling Phage Plaque Sizes - Experiment One.

8/13/13

- Started approximately 20mL of E coli B liquid culture overnight.

8/14/13

- Performed mutagenesis and spot test for 8.14 Mutagen Concentration Test - Seventh Protocol.

- Performed spot test for T1 propagation. Spotted 5uL of -2 through -7 dilutions.

8/15/13

- T1 propagation revealed plaques on each of the tested dilutions, suggesting that this propagated T1 phage stock has a titer of at least 10⁹ pfu/mL -> more accurate spot test / titer needed to determine the exact phage concentration.

- Started approximately 30mL of E coli B liquid culture overnight.

8/16/13

- Started 8.16 Modeling Phage Plaque Sizes - Experiment One.

- Phage Purification team performed the CsCl gradient for 8.14 Mutagen Concentration Test - Seventh Protocol.

- Started approximately 10 mL of E coli B liquid culture overnight.