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From 2013.igem.org

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• iGEM 2013

Contents 1 5/27/13 2 5/29/13 3 5/31/13 4 June 4.1 6/3/13 4.2 6/5/13 4.3 6/7/13

5/27/13

5/29/13

Today we received our kit containing E. coli B, T1, T2, and T3. We streaked out some of the E. coli B on an LB plate in preparation to grow it in a flask and archive it in the freezer.

Today we wanted to see if we can transfer our current experiment from using E. coli W3110 as the host to E. coli B as the host. We set up multiple experiments.

1. We spot tested the following phage samples on W3110 and B: T1, T2, T3, T4Do stock, T4 mutated stock, T4-UV one-plaque plate harvest, T4-UV-mutated one-plaque plate harvest.
2. We did a dilution series on the T1 stock to see how concentrated it is. We will need to know the PFUs in order to grow a successful liquid culture and mass produce it for our large phage amplification procedure.
3. We also infected two samples of E. coli B, each with 10 uL of 10^-6 T4Do to observe plaque formation.

We archived two cryo-tubes of E. coli B by mixing 600 uL each of overnight E. coli B and 40% glycerol. These two tubes will be stored at -80C.

5/31/13

I took out the plates from last time's experiments.

1. The spot test showed that all seven samples cleared both E. coli B and W3110.
2. However, our dilution series for T1 did not work. All of the plates appeared to show only bacterial lawns. The plates were not observed after a 24 hour incubation, but 48 hours later there were only lawns on all the plates.
3. The 10^-6 T4Do plates produced excellent plaques, confirming that T4Do infects E. coli B.

Also, today Jade taught me how to set up the website- so I worked on that.

June

6/3/13

KS- I spent most of today getting the website in order and organized for future experiments to be entered in easily!

6/5/13

6/7/13