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From 2013.igem.org

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  • Overview
  • Background
  • Experimental Design
• Notebook
  • Overview
  • Phage Team
  • Cholera Team
• Results
  • Overview
  • Judging Criteria
  • Experimental
  • Modeling
  • Parts Submitted to the Registry
  • A Taste of Toronto
• Human Practices
  • Overview
  • Orem Public Library Visit
  • The Adventure of Baxter Bacteria
• Safety
• Attributions & Collaborations
  • Attributions & Acknowledgements
  • Collaborations
• iGEM 2013

Cholera Detection May - June Notebook
Cholera Detection March-April May-June July-August September-October	May 1 - May 12 We attempted to amplify Cro through pcr and cut it out of a gel. Cro will be used to induce the lytic cycle of lambda. We believe that when there is an abundance of cro, there will be an increased lysis of lambda. Daily log
	May 13 - May 26 Add stuff here Daily log
	May 27 - June 9 Add description! Daily log
	June 10 - June 23 With the correct primers, DMSO, and fresh template, we performed a successful PCR reaction of CRO. We continued the cloning process by digesting pIG12 and CRO, ligating them together, and transforming the ligated plasmid into DH5a E.Coli. Daily log
	June 24 - June 30 Add description! Daily log