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Team:USTC CHINA/Notebook/Protocols
From 2013.igem.org
Gel Extraction
DNA digestion
Single Digestion
Double Digestion
Colony PCR
PCR
Ligation
Plasmid mini-prep
Transformation
The transformation of Bacillus subtilis
Overlap PCR
Extracting plasmids
Extracting plasmids from gram-positive bacterium
Extracting plasmids from gram-negative bacterium
Expression of proteins in Bacillus subtilis
Expression of proteins in Escherichia coli
Concentrating proteins
ELISA
Sample analysis
LB media
10*S base
HS media
LS media
2*YT media
Constitutive promoter measurements
plac promoter response to IPTG or Glucose
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