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Team:BYU Provo/Notebook/SmallPhage/Springexp/Period4/Dailylog
From 2013.igem.org
| Small Phage May - June Notebook: June 17 - June 30 Daily Log
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6/17/13 - Plated 5 controls and 50 selection plates (500ug at -2) as part of selection 1 in 6.12 Mutagen Concentration Test - Perfected Protocol
5/28/13 - Started three 8mL E coli BL21 liquid culture at around 4pm.
5/29/13 - Continued 5.20 Mutagen Concentration Experiment - Prepared sample for sequencing. This is done as part of 5.20 T7 Minor Capsid Protein PCR - Dr. Studier responded to our email today! We also received the E coli stains containing plasmids with clone T7 genes. WE CAN START SITE DIRECTED MUTAGENESIS SOON!
5/30/13 - Plates from yesterday are taken out of incubation at around 4:00pm
5/31/13 - Discussed results for 5.20 Mutagen Concentration Experiment -> will need to try this one more time with minor adjustment to experimental design: plate -4 dilution using x6 and x8 top agar. - Discussed plans for next week. - Made new LB and x6 top agar.
6/2/13 - Made about 20ml of BL21 overnight
6/3/13 - Made new LB plates - Plated -4 titer on x6 and x8 plates for 5.20 Mutagen Concentration Experiment
6/5/13 - Discussed plans for the selection process of 5.20 Mutagen Concentration Experiment and calculated the needed volume of agar, overnight, and phage - Made 500mL x8 agar - Performed dilution series to generate enough 200ug -4 phage stock for selection.
6/6/13 - Started approximately 50mL of BL21 liquid culture overnight.
6/7/13 - Performed the selection process of 5.20 Mutagen Concentration Experiment. Specifically, we plated 45 plates of 200ug mutagen, -4 phage dilution using x8 agar. 5 plates of 0ug, -3 phage dilution were also done a control.
6/9/13 - Started 21mL of BL21 overnight.
6/10/13 - Made 1250mL of x8 top agar - Attempted to amplify phage from larger plaques in 5.20 Mutagen Concentration Experiment
6/11/13 - Started approximately 70mL of BL21 liquid culture overnight
6/12/13 - Perfected protocol for applying mutagen to phage - Started 6.12 Mutagen Concentration Test - Perfected Protocol
6/13/13 - Started approximately 30mL of BL21 liquid culture overnight.
6/14/13 - Titered the mutagenesis product from Wednesday as part of 6.12 Mutagen Concentration Test - Perfected Protocol
6/16/13 - Started approximately 100mL of BL21 liquid culture overnight.
6/17/13 - Plated 5 controls and 50 selection plates (500ug at -2) as part of selection 1 in 6.12 Mutagen Concentration Test - Perfected Protocol
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