Home

Team

Project

Parts

Protocol

Notebook

Results

Safety

Human Practice

Results

1.IL10recepter We inserted Il10receptere gene into vecter

1 transformation Figure 1transformation of Il10recepter α Figure2 transformation of Il10recepter β 2 .culture on the LB nutrient medium and get colonies. 3 colony PCR The molecular weight of the α chain is 1734bp. The molecular weight of the α chain is 975bp Figure3 Il10recepter α colony PCR Figure4 Il10recepter β colony PCR 4 restriction enzyme processing PCR production 5 infusion 6 .transformation 7 culture on the LB nutrient medium and get colonies. 8 colony PCR The molecular weight of the vector is a 4000 ~ 5000bp. We were not able to confirm the vector by colony PCR.

2 STAT3 We inserted stat3 gene into vector 1 transformation Figure5 transformation of STAT3 2 .culture on the LB nutrient medium and get colonies. 3 colony PCR The molecular weight of the α chain is 2307bp. Figure6 STAT3 colony PCR 4 restriction enzyme processing PCR production 5 infusion 6 .transformation 7 culture on the LB nutrient medium and get colonies. 8 colony PCR The molecular weight of the vector is a 4000 ~ 5000bp. We were not able to confirm the vector by colony PCR.

3 GFP+HlyA We inserted GFP gene and HlyA into vecter

1 transformation Figure 1transformation of GFP. Figure2 transformation of HlyA. 2 .culture on the LB nutrient medium and get colonies. 3 colony PCR The molecular weight of the α chain is 720bp. The molecular weight of the α chain is 186bp Figure3 Il10recepter GFP PCR Figure4 Il10recepter HlyA PCR 4 restriction enzyme processing PCR production 5 infusion 6 .transformation 7 culture on the LB nutrient medium and get colonies. 8 colony PCR The molecular weight of the vector is a 2000~3000bp. We were not able to confirm the vector by colony PCR.