Team:SydneyUni Australia/Modelling Conclusion

From 2013.igem.org

Conclusions:

• In our model we find that 1mM of DCA is removed from solution within roughly 50 minutes when the DCA degrading cells are at a concentration of 2E8 cells/mL (graph 1 and graph 5).

• It is also evident that bacterial growth can occur (graph 4, graph 8 and graph 9). This growth is due to the degradation of DCA to glycolate. We can also see that that bacterial growth correlates with glycolate accumulation (by comparing graph 6 and graph 9).

• The cytotoxic metabolic intermediate chloroactealdehyde does not accumulate to a significant concentration in either of the pathways and is consistently at a negligibly low concentration. We can see that chloroacetaldehyde reaches a maximum concentration of roughly 0.2 mM in both pathways (graph 3 and graph 7). Chloroacetaldehyde is apparently metabolised very quickly; this concentration maximum is short lived, peaks at roughly 0.03 seconds and returns back to 0 mM by 0.5 seconds. It is expected that chloroacetaldehyde toxicity will not be a problem in our engineered cells.

• We also conclude that the two pathways remove DCA at the same rate (by comparing graph 1 and graph 5).

References:

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[7] CyberCell Database

[10] http://www.dtsc.ca.gov/AssessingRisk/Upload/12dca.pdf

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