Exeter/10 July 2013

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Revision as of 11:15, 16 July 2013 by Fentwistle (Talk | contribs)

Morning

MiniPrep of the following Parts:

- BBa_K322122, cyan pigment

- BBa_K592012, magenta pigment

- BBa_K592010, yellow pigment

Plasmids extracted are now in storage at -20oC.


Afternoon

Liquid cultures of the blue light module and magenta pigment

- BBa_K592010, yellow pigment - BBa_K592012, magenta pigment - BBa_B0015, a terminator - BBa_K592005, FixJ protein intermediate - BBa_K592004, blue light sensor

Retry of BBa_B0034 transformation

Normal transformation protocol from 4/7/13 followed. Made certain we were using the right antibiotic plates!

(Next day, results of BBa_B0034 transformation)

We made two plates of BBa_B0034, each plate had 7 colonies. Our RFP control had 137 colonies.