Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period2/Dailylog

7/16/13

- Started approximately 60mL of BL21 liquid culture overnight.

7/17/13

- The titer from 7.15 was too low to start an actual round of mutagenesis. One more round of propagation was performed for Team:BYU_Provo/Notebook/SmallPhage/Summer/Period1/Exp/7.13_Phage_Propagation

- Do we want to talk about this round of mutagenesis? phage stock 80e6. 1.5mL of E coli, 90uL of phage.

7/3/13

- Performed spot test for 6.27 Mutagen Concentration Test - Third Protocol to determine phage viability. 5.3 stock and phage from 6.12 Mutagen Concentration Test - Second Protocol were used as positive control.

- Worked on the introduction for our outreach presentation.

7/7/13

- Started 60mL of BL21 liquid culture overnight.

7/8/13

- Made 750mL of x8 top agar.

- We started our fourth round of mutagenesis in 7.8 Mutagen Concentration Test - Fourth Protocol

7/9/13

- Outreach @ Orem Public Library!

- Started approximately 20mL of E coli BL21 liquid culture overnight.

7/10/13

- Performed titer to determine mutated phage concentration for 7.8 Mutagen Concentration Test - Fourth Protocol

7/11/13

- Started approximately 10mL of E coli BL21 liquid culture overnight.

7/12/13

- Performed spot test to confirm the titer result from July 10th. Detail procedure listed in 7.8 Mutagen Concentration Test - Fourth Protocol.

- Started approximately 6 mL of E coli liquid culture overnight.

7/13/13

- Set up the phage liquid culture for propagation in 7.13 Phage Propagation Experiment.

7/14/13

- Started approximately 30mL of E coli BL21 liquid culture overnight.